Gadd45, a p53-Responsive Stress Protein, Modifies DNA Accessibility on Damaged Chromatin

France Carrier; Philippe T. Georgel; Philippe Pourquier; Mellissa Blake; H. Udo Kontny; Michael J. Antinore; Marzia Gariboldi; Timothy G. Myers; John N. Weinstein; Yves Pommier; Albert J. Fornace

首页> 外文期刊>Molecular and Cellular Biology >Gadd45, a p53-Responsive Stress Protein, Modifies DNA Accessibility on Damaged Chromatin

【24h】

Gadd45, a p53-Responsive Stress Protein, Modifies DNA Accessibility on Damaged Chromatin

机译：Gadd45是一种p53反应性应激蛋白，可修饰受损染色质的DNA可及性

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

This report demonstrates that Gadd45, a p53-responsive stress protein, can facilitate topoisomerase relaxing and cleavage activity in the presence of core histones. A correlation between reduced expression of Gadd45 and increased resistance to topoisomerase I and topoisomerase II inhibitors in a variety of human cell lines was also found. Gadd45 could potentially mediate this effect by destabilizing histone-DNA interactions since it was found to interact directly with the four core histones. To evaluate this possibility, we investigated the effect of Gadd45 on preassembled mononucleosomes. Our data indicate that Gadd45 directly associates with mononucleosomes that have been altered by histone acetylation or UV radiation. This interaction resulted in increased DNase I accessibility on hyperacetylated mononucleosomes and substantial reduction of T4 endonuclease V accessibility to cyclobutane pyrimidine dimers on UV-irradiated mononucleosomes but not on naked DNA. Both histone acetylation and UV radiation are thought to destabilize the nucleosomal structure. Hence, these results imply that Gadd45 can recognize an altered chromatin state and modulate DNA accessibility to cellular proteins.

机译：该报告证明，在核心组蛋白存在下，p53反应性应激蛋白Gadd45可以促进拓扑异构酶的松弛和裂解活性。还发现在多种人类细胞系中，Gadd45的表达降低与对拓扑异构酶I和拓扑异构酶II抑制剂的耐药性增加之间存在相关性。由于发现Gadd45与四个核心组蛋白直接相互作用，因此它可能通过破坏组蛋白与DNA的相互作用来介导这种作用。为了评估这种可能性，我们研究了Gadd45对预组装单核小体的影响。我们的数据表明，Gadd45与已被组蛋白乙酰化或UV辐射改变的单核小体直接缔合。这种相互作用导致在超乙酰化的单核小体上增加DNase I的可及性，并在紫外线照射的单核小体上显着降低T4核酸内切酶V对环丁烷嘧啶二聚体的可及性，而在裸露的DNA上则没有。组蛋白乙酰化和紫外线辐射都被认为会破坏核小体结构的稳定性。因此，这些结果表明，Gadd45可以识别染色质状态的改变并调节DNA对细胞蛋白的可及性。

著录项

来源
《Molecular and Cellular Biology》 |1999年第3期|共13页
作者
France Carrier; Philippe T. Georgel; Philippe Pourquier; Mellissa Blake; H. Udo Kontny; Michael J. Antinore; Marzia Gariboldi; Timothy G. Myers; John N. Weinstein; Yves Pommier; Albert J. Fornace;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Nucleosome binding proteins potentiate ATM activation and DNA damage response by modifying chromatin. [J] . Gerlitz G, Bustin M Cell cycle . 2009,第11期

机译：核小体结合蛋白通过修饰染色质增强ATM活化和DNA损伤反应。
2. Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress [J] . HanWang, PornpimolTipthara, LeiZhu, International journal of proteomics . 2012,第1期

机译：SILAC蛋白质组学分析揭示了染色质相关蛋白表现出在DNA损伤胁迫下对生长适应性要求的高可能性
3. CCAAT/enhancer binding protein beta (C/EBP beta) regulates the transcription of growth arrest and DNA damage-inducible protein 45 beta (GADD45 beta) in articular chondrocytes [J] . Shimada Hirofumi, Otero Miguel, Tsuchimochi Kaneyuki, Pathology Research and Practice . 2016,第4期

机译：CCAAT /增强子结合蛋白beta（C / EBP beta）调节关节软骨细胞中生长停滞和DNA损伤诱导蛋白45 beta（GADD45 beta）的转录
4. Growth arrest and DNA damage inducible gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology [C] . Pin-Pin Hsu, Benjamin Chen, Suli Yuan, IEEE Engineering in Medicine and Biology Annual Conference . 1999

机译：通过使用DNA微阵列技术鉴定的鉴定，通过使用DNA微阵列技术鉴定的层状剪切应力诱导生长停滞和DNA损伤诱导基因45（GADD45）
5. Organometallic photonucleases. I. DNA cleavage by the photolysis of cyclopentadienyl metal complexes: Mechanistic studies and sequence selectivity of strand scission by CpW(CO)3CH3. II. Footprinting of chromatin structure and chromatin remodeling by SWI/SNF utilizing CpW(CO)3CH3. III. Dual mechanisms of DNA damage by MoX(eta3-allyl)(CO)3(phen) complexes. [D] . Downs, Jennifer. 2005

机译：有机金属光核酸酶。 I.通过环戊二烯基金属配合物的光解来切割DNA：通过CpW（CO）3CH3进行断裂的机理研究和序列选择性。二。 SWI / SNF利用CpW（CO）3CH3进行染色质结构足迹测定和染色质重塑。三， MoX（eta3-烯丙基）（CO）3（phen）复合物对DNA的双重破坏机制。
6. Gadd45 a p53-Responsive Stress Protein Modifies DNA Accessibility on Damaged Chromatin [O] . France Carrier, Philippe T. Georgel, Philippe Pourquier, 1999

机译：Gadd45是一种p53反应性应激蛋白可修饰受损染色质的DNA可及性
7. Gadd45, a p53-Responsive Stress Protein, Modifies DNA Accessibility on Damaged Chromatin [O] . Carrier, France, Georgel, Philippe T., Pourquier, Philippe, 1999

机译：Gadd45是一种p53反应性应激蛋白，可修饰受损染色质的DNA可及性

Gadd45, a p53-Responsive Stress Protein, Modifies DNA Accessibility on Damaged Chromatin

摘要

著录项

相似文献

相关主题

期刊订阅