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首页> 外文期刊>Molecular and Cellular Biology >Glycogen Synthase Phosphatase Interacts with Heat Shock Factor To Activate CUP1 Gene Transcription inSaccharomyces cerevisiae
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Glycogen Synthase Phosphatase Interacts with Heat Shock Factor To Activate CUP1 Gene Transcription inSaccharomyces cerevisiae

机译：糖原合酶磷酸酶与热激因子相互作用以激活酿酒酵母中的CUP1基因转录。

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Upon heat shock, transcription of many stress-inducible genes is rapidly and dramatically stimulated by heat shock factor (HSF). A central region of the yeast HSF (designated HSFrr for “repression region”) was previously identified and proposed to be involved in repressing the activation domain under non-heat-shock conditions. Here, we used the phage display system to isolate proteins that interact with HSFrr. This should identify factors that modulate HSF activity or directly participate in HSF-mediated transcriptional activation. We constructed a randomly sheared yeast genomic library to express yeast proteins on the surface of λ phage. HSFrr binding phages were selected by cycles of affinity chromatography. DNA sequencing identified an HSFrr-interacting phage that contains theGAC1 gene. The GAC1 gene encodes the regulatory subunit for a type 1 serine/threonine phosphoprotein phosphatase, Glc7. Both gac1 and glc7 mutations had little effect on HSF activation of gene transcription of two heat shock genes,SSA4 and HSP82. In contrast, heat shock induction of CUP1 gene expression was completely abolished in a glc7 mutant and reduced in a gac1 mutant. The results demonstrate that the Glc7 phosphatase and its Gac1 regulatory subunit play positive roles in HSF activation ofCUP1 transcription.
机译：热激后，热激因子（HSF）会迅速显着刺激许多应激诱导基因的转录。酵母HSF的中央区域（称为“阻抑区域”指定为HSFrr）先前已确定，并建议在非热激条件下参与抑制激活结构域。在这里，我们使用噬菌体展示系统来分离与HSFrr相互作用的蛋白质。这应确定调节HSF活性或直接参与HSF介导的转录激活的因子。我们构建了一个随机剪切的酵母基因组文库，以在λ噬菌体表面表达酵母蛋白。通过亲和层析循环选择HSFrr结合噬菌体。 DNA测序鉴定出一个与HSFrr相互作用的噬菌体，其中含有 GAC1 基因。 GAC1 基因编码1型丝氨酸/苏氨酸磷酸蛋白磷酸酶Glc7的调节亚基。 gac1 和 glc7 突变对热休克蛋白激活两个热休克基因 SSA4 和 HSP82 。相反，在 glc7 突变体中完全消除了 CUP1 基因表达的热激诱导，而在 gac1 突变体中减少了这种表达。结果表明，Glc7磷酸酶及其Gac1调节亚基在HSF激活 CUP1 转录中发挥积极作用。

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