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首页> 外文期刊>Molecular and Cellular Biology >The Adeno-Associated Virus Type 2 Rep Protein Regulates RNA Processing via Interaction with the Transcription Template
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The Adeno-Associated Virus Type 2 Rep Protein Regulates RNA Processing via Interaction with the Transcription Template

机译：腺相关病毒2型Rep蛋白通过与转录模板相互作用调节RNA加工。

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The adeno-associated virus type 2 (AAV) large Rep proteins can act to increase the ratio of spliced to unspliced AAV RNA when they are targeted to the transcription template via a Rep binding element. The required Rep binding site is both location and orientation independent; however, Rep enhancement decreases as the distance between the promoter and the intron of the affected transcription unit increases. Only the AAV intron and an extended polyadenylation site must remain for the AAV transcription unit to manifest responsiveness to Rep. A number of promoters, when driving the AAV capsid gene transcription unit, were responsive to targeted Rep, though to various degrees. Transactivation of transcription initiation is not sufficient for the enhancement of RNA processing, because activation of the P40 transcription unit by other activators targeted to this transcription template did not result in enhancement of the ratio of spliced to unspliced AAV RNA. These results suggest that Rep may act as a trans regulator of RNA processing by modulating such functions coupled to RNA polymerase II (RNA pol II) transcription, perhaps by affecting the composition of the transcription complex either prior to or during elongation. These results reveal another way in which gene expression can be regulated by trans-acting proteins and help explain an important feature of the parvovirus life cycle.
机译：当腺相关病毒2型（AAV）大Rep蛋白通过Rep结合元件靶向转录模板时，可起到增加剪接的AAV RNA与未剪接的AAV RNA比例的作用。所需的Rep结合位点与位置和方向无关;然而，随着启动子和受影响转录单位的内含子之间的距离增加，Rep增强会降低。只有AAV内含子和延伸的聚腺苷酸化位点必须保留，AAV转录单元才能表现出对Rep的响应性。驱动AAV衣壳基因转录单元时，许多启动子对目标Rep都有响应，尽管程度不同。转录起始的反式激活不足以增强RNA加工，因为靶向该转录模板的其他激活剂激活P40转录单位不会导致剪接的AAV RNA与未剪接的AAV RNA的比例增加。这些结果表明，Rep可能通过调节与RNA聚合酶II（RNA pol II）转录相关的功能来充当RNA加工的 trans 调节剂，可能是通过影响转录复合物的组成，在转录之前或之后在伸长过程中。这些结果揭示了可通过 trans 作用蛋白调节基因表达的另一种方式，并有助于解释细小病毒生命周期的重要特征。

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