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首页> 外文期刊>Molecular and Cellular Biology >Stability of maternal mRNA in Xenopus embryos: role of transcription and translation.
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Stability of maternal mRNA in Xenopus embryos: role of transcription and translation.

机译：爪蟾胚胎中母体mRNA的稳定性：转录和翻译的作用。

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The first 12 cell divisions of Xenopus laevis embryos do not require gene transcription. This means that the regulation of gene expression during this period is controlled at post transcriptional levels and makes Xenopus early development a potentially interesting biological system with which to study the mechanisms involved. We describe here the stability characteristics of several maternal Xenopus mRNAs which are deadenylated soon after fertilisation (J. Paris and M. Philippe, Dev. Biol., in press). We show that these mRNAs were only degraded in the embryo after the midblastula transition (MBT), when gene transcription was initiated. The kinetics with which the deadenylated maternal mRNAs decreased in the post-MBT embryos showed sequence specificity. The degradation of these mRNAs after the MBT was inhibited by cycloheximide but was not affected by dactinomycin. Therefore, the destabilization of these mRNAs does not appear to be initiated by new embryonic gene transcripts. Sequence comparisons of the 3' untranslated region of these mRNAs identified several motifs which may be involved in the posttranscriptional control of these gene products.
机译：非洲爪蟾胚胎的前12个细胞分裂不需要基因转录。这意味着在此期间基因表达的调控被控制在转录后水平，并使非洲爪蟾的早期发育成为潜在有趣的生物系统，用以研究所涉及的机制。我们在这里描述了受精后很快被去烯基化的几种母本爪蟾mRNA的稳定性特征（J. Paris and M. Philippe，Dev。Biol。，印刷中）。我们表明这些mRNA仅在胚泡过渡期（MBT），当基因转录开始后在胚胎中降解。 MBT后胚胎中的去甲腺苷酸母体mRNA减少的动力学显示了序列特异性。 MBT后这些基因的降解被环己酰亚胺抑制，但不受放线菌素的影响。因此，这些mRNA的不稳定似乎不是由新的胚胎基因转录本引发的。这些mRNA的3'非翻译区的序列比较确定了可能与这些基因产物的转录后控制有关的几个基序。

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