...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Proliferating cell nuclear antigen-dependent abasic site repair in Xenopus laevis oocytes: an alternative pathway of base excision DNA repair.
【24h】

Proliferating cell nuclear antigen-dependent abasic site repair in Xenopus laevis oocytes: an alternative pathway of base excision DNA repair.

机译：非洲爪蟾卵母细胞中增殖细胞核抗原依赖性无碱基位点修复：碱基切除DNA修复的另一种途径。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

DNA damage frequently leads to the production of apurinic/apyrimidinic (AP) sites, which are presumed to be repaired through the base excision pathway. For detailed analyses of this repair mechanism, a synthetic analog of an AP site, 3-hydroxy-2-hydroxymethyltetrahydrofuran (tetrahydrofuran), has been employed in a model system. Tetrahydrofuran residues are efficiently repaired in a Xenopus laevis oocyte extract in which most repair events involve ATP-dependent incorporation of no more than four nucleotides (Y. Matsumoto and D. F. Bogenhagen, Mol. Cell. Biol. 9:3750-3757, 1989; Y. Matsumoto and D. F. Bogenhagen, Mol. Cell. Biol. 11:4441-4447, 1991). Using a series of column chromatography procedures to fractionate X. laevis ovarian extracts, we developed a reconstituted system of tetrahydrofuran repair with five fractions, three of which were purified to near homogeneity: proliferating cell nuclear antigen (PCNA), AP endonuclease, and DNA polymerase delta. This PCNA-dependent system repaired natural AP sites as well as tetrahydrofuran residues. DNA polymerase beta was able to replace DNA polymerase delta only for repair of natural AP sites in a reaction that did not require PCNA. DNA polymerase alpha did not support repair of either type of AP site. This result indicates that AP sites can be repaired by two distinct pathways, the PCNA-dependent pathway and the DNA polymerase beta-dependent pathway.
机译：DNA损伤经常导致产生嘌呤/嘧啶（AP）位点，据推测这些位点可通过碱基切除途径修复。为了对该修复机制进行详细分析，已在模型系统中使用了AP部位的合成类似物3-羟基-2-羟甲基四氢呋喃（四氢呋喃）。四氢呋喃残基在非洲爪蟾卵母细胞提取物中得到有效修复，其中大多数修复事件涉及不超过四个核苷酸的ATP依赖性掺入（Y. Matsumoto和DF Bogenhagen，Mol。Cell。Biol。9：3750-3757，1989; Y Matsumoto和DF Bogenhagen，Mol.Cell.Biol.11：4441-4447，1991）。我们使用一系列的柱色谱程序对X.laevis卵巢提取物进行分级分离，我们开发了一种重构的四氢呋喃修复系统，其中包含五个馏分，其中三个被纯化至接近均一：增殖细胞核抗原（PCNA），AP核酸内切酶和DNA聚合酶三角洲。这种依赖PCNA的系统修复了天然AP位点以及四氢呋喃残基。在不需要PCNA的反应中，DNA聚合酶beta只能代替DNA聚合酶δ来修复天然AP位点。 DNA聚合酶α不支持修复任何一种AP位点。该结果表明AP位点可以通过两种不同的途径修复，即PCNA依赖性途径和DNA聚合酶β依赖性途径。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号