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首页> 外文期刊>Molecular and Cellular Biology >Regulation of RAG-2 protein expression in avian thymocytes.
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Regulation of RAG-2 protein expression in avian thymocytes.

机译：RAG-2蛋白在禽胸腺细胞中的表达调控。

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The recombinase-activating genes, RAG-1 and RAG-2, have been shown to be necessary to initiate the process of V(D)J recombination during the ontogeny of lymphocytes. While much is known about the end products of this rearrangement process, little is known about the function or regulation of the components of the recombinase system. To this end, we have generated a monoclonal antibody to the chicken RAG-2 protein. Chicken thymocytes were found to express high levels of RAG-2, part of which is phosphorylated. Within thymocytes, RAG-2 is expressed primarily within the nucleus. RAG-2 protein levels are high in the CD4- CD8- and CD4+ CD8+ immature thymocytes but absent at the single-positive CD4+ CD8- or CD4- CD8+ stage of thymocyte development. Mitogenic stimulation of thymocytes with phorbol myristate acetate and ionomycin results in down-regulation of RAG-2 expression. Consistent with these data, in vivo levels of RAG-2 are markedly lower in proliferating thymocytes than in smaller, G0/G1 cells. Down-regulation of RAG-2 expression appears to occur before cells enter S phase, suggesting that RAG-2 function may be limited to noncycling cells.
机译：重组酶激活基因RAG-1和RAG-2已被证明是在淋巴细胞发生过程中启动V（D）J重组过程所必需的。尽管对该重排过程的最终产物知之甚少，但对于重组酶系统各组分的功能或调节却知之甚少。为此，我们已经产生了针对鸡RAG-2蛋白的单克隆抗体。发现鸡胸腺细胞表达高水平的RAG-2，其中一部分被磷酸化。在胸腺细胞内，RAG-2主要在细胞核内表达。 RAG-2蛋白水平在CD4-CD8-和CD4 + CD8 +未成熟胸腺细胞中较高，但在胸腺细胞发育的单阳性CD4 + CD8-或CD4- CD8 +阶段则不存在。佛波肉豆蔻酸酯乙酸盐和离子霉素对胸腺细胞的有丝分裂刺激导致RAG-2表达的下调。与这些数据一致，增殖的胸腺细胞中的RAG-2体内水平明显低于较小的G0 / G1细胞。 RAG-2表达的下调似乎发生在细胞进入S期之前，这表明RAG-2的功能可能仅限于非循环细胞。

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