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首页> 外文期刊>Molecular and Cellular Biology >Chromosomal DNA replication initiates at the same origins in meiosis and mitosis.
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Chromosomal DNA replication initiates at the same origins in meiosis and mitosis.

机译：染色体DNA复制起始于减数分裂和有丝分裂的相同起源。

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Autonomously replicating sequence (ARS) elements are identified by their ability to promote high-frequency transformation and extrachromosomal replication of plasmids in the yeast Saccharomyces cerevisiae. Six of the 14 ARS elements present in a 200-kb region of Saccharomyces cerevisiae chromosome III are mitotic chromosomal replication origins. The unexpected observation that eight ARS elements do not function at detectable levels as chromosomal replication origins during mitotic growth suggested that these ARS elements may function as chromosomal origins during premeiotic S phase. Two-dimensional agarose gel electrophoresis was used to map premeiotic replication origins in a 100-kb segment of chromosome III between HML and CEN3. The pattern of origin usage in premeiotic S phase was identical to that in mitotic S phase, with the possible exception of ARS308, which is an inefficient mitotic origin associated with CEN3. CEN3 was found to replicate during premeiotic S phase, demonstrating that the failure of sister chromatids to disjoin during the meiosis I division is not due to unreplicated centromeres. No origins were found in the DNA fragments without ARS function. Thus, in both mitosis and meiosis, chromosomal replication origins are coincident with ARS elements but not all ARS elements have chromosomal origin function. The efficiency of origin use and the patterns of replication termination are similar in meiosis and in mitosis. DNA replication termination occurs over a broad distance between active origins.
机译：自主复制序列（ARS）元件通过其在啤酒酵母中促进质粒的高频转化和染色体外复制的能力来鉴定。存在于啤酒酵母第200号染色体的200 kb区域中的14种ARS元素中有6种是有丝分裂染色体复制起点。出乎意料的观察表明，八个ARS元素在有丝分裂生长过程中不能作为染色体复制起点发挥可检测的水平，这表明这些ARS元素在减数分裂S期可能作为染色体起源。二维琼脂糖凝胶电泳用于在HML和CEN3之间的III号染色体的100 kb片段中绘制减数分裂前复制起点。减数分裂前S期的起源使用模式与有丝分裂S期的相同，可能是ARS308例外，后者是与CEN3相关的低效率的有丝分裂起源。 CEN3被发现在减数分裂前S期复制，表明姊妹染色单体在减数分裂I分裂期间不能分离的原因并非归因于着丝粒的复制。没有ARS功能的DNA片段中未发现任何来源。因此，在有丝分裂和减数分裂中，染色体复制起点都与ARS元素一致，但并非所有ARS元素都具有染色体起源功能。在减数分裂和有丝分裂中，来源使用的效率和复制终止的模式相似。 DNA复制终止发生在活跃起点之间的较远距离上。

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