Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B' protein.

J Tang; N Abovich; M Rosbash

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Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B' protein.

机译：编码U2小核糖核糖核蛋白颗粒B“蛋白的酵母基因的鉴定和表征。

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The inessential yeast gene MUD2 encodes a protein factor that contributes to U1 small nuclear ribonucleoprotein particle (snRNP)-pre-mRNA complex (commitment complex) formation. To identify other genes that contribute to this early splicing step, we performed a synthetic lethal screen with a MUD2 deletion strain. The first characterized gene from this screen, MSL1 (MUD synthetic lethal 1), encodes the yeast homolog of the well studied mammalian snRNP protein U2B". The yeast protein (YU2B") is a component of yeast U2 snRNP, and it is related to other members of the UIA-U2B" family, the human U2B" protein, the human U1A protein, and the yeast U1A protein. It binds in vitro to its RNA target, U2 snRNA stem-loop IV, without a protein cofactor, and the target resembles more closely the U1 snRNA binding site of the human U1A protein than it does the U2 snRNA binding site of human U2B". Surprisingly, the YU2B" protein lacks a C-terminal RNA binding domain, which is conserved in all other family members. Possible functional and evolutionary relationships among these proteins are discussed.

机译：非必需酵母基因MUD2编码有助于U1小核糖核蛋白颗粒（snRNP）-pre-mRNA复合物（承诺复合物）形成的蛋白质因子。为了鉴定有助于此早期剪接步骤的其他基因，我们对MUD2缺失菌株进行了合成致死筛选。此筛选中的第一个特征基因MSL1（MUD合成致死1）编码经过充分研究的哺乳动物snRNP蛋白U2B“的酵母同源物。酵母蛋白（YU2B”）是酵母U2 snRNP的组成部分，与UIA-U2B”家族的其他成员，人U2B”蛋白，人U1A蛋白和酵母U1A蛋白。它在没有蛋白质辅因子的情况下与它的RNA靶标U2 snRNA茎环IV体外结合，并且该靶标与人U1A蛋白的U1 snRNA结合位点比与人U2B的U2 snRNA结合位点更相似。令人惊讶地，YU2B”蛋白缺乏C末端RNA结合结构域，该结构域在所有其他家族成员中都是保守的。讨论了这些蛋白质之间可能的功能和进化关系。

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《Molecular and Cellular Biology》 |1996年第6期|共9页
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J Tang; N Abovich; M Rosbash;
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1. Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B" protein. [J] . J Tang, N Abovich, M Rosbash Molecular and Cellular Biology . 1996,第6期

机译：编码U2小核糖核糖核蛋白颗粒B“蛋白的酵母基因的鉴定和表征。
2. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding. [J] . N Abovich, P Legrain, M Rosbash Molecular and Cellular Biology . 1990,第12期

机译：酵母PRP6基因编码U4 / U6小核糖核蛋白颗粒（snRNP）蛋白，PRP9基因编码U2 snRNP结合所需的蛋白。
3. Recognition of U1 and U2 small nuclear RNAs can be altered by a 5-amino-acid segment in the U2 small nuclear ribonucleoprotein particle (snRNP) B" protein and through interactions with U2 snRNP-A' protein. [J] . R C Bentley, J D Keene Molecular and Cellular Biology . 1991,第4期

机译：U1和U2小核RNA的识别可以通过U2小核糖核蛋白颗粒（snRNP）B“蛋白中的5个氨基酸段以及与U2 snRNP-A'蛋白的相互作用来改变。
4. An Efficient Strategy for the Identification of UV-induced Protein-RNA Crosslinks in native Ribonucleoprotein Particles based on ESI-MS [C] . Florian Richter, He-Hsuan Hsiao, Xiao Luo, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：基于ESI-MS的天然核糖核蛋白颗粒中鉴定UV诱导蛋白-RNA交联的有效策略
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6. Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B protein. [O] . J Tang, N Abovich, M Rosbash 1996

机译：编码U2小核糖核糖核蛋白颗粒B蛋白的酵母基因的鉴定和表征。
7. Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B" protein. [O] . Tang, J, Abovich, N, Rosbash, M 1996

机译：编码U2小核糖核糖核蛋白颗粒B“蛋白的酵母基因的鉴定和表征。
8. Cloning and Characterization of Genes Encoding Proteins Involved in the TerminalStage of Vesicular Traffic in the Yeast 'Saccharomyces cerevisiae' [R] . Aalto, M. 1995

机译：酵母'酿酒酵母'中水泡交通终末期蛋白质编码基因的克隆与鉴定

Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B' protein.

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