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首页> 外文期刊>Molecular and Cellular Biology >Argonaute Protein in the Early Divergent Eukaryote Trypanosoma brucei: Control of Small Interfering RNA Accumulation and Retroposon Transcript Abundance
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Argonaute Protein in the Early Divergent Eukaryote Trypanosoma brucei: Control of Small Interfering RNA Accumulation and Retroposon Transcript Abundance

机译：早期发散的真核锥虫布鲁氏菌中的Argonaute蛋白：小干扰RNA积累和反转录子转录本丰度的控制。

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Members of the Argonaute protein family have been linked through a combination of genetic and biochemical studies to RNA interference (RNAi) and related phenomena. Here, we describe the characterization of the first Argonaute protein (AGO1) in Trypanosoma brucei, the earliest divergent eukaryote where RNAi has been described so far. AGO1 is predominantly cytoplasmic and is found in a ribonucleoprotein particle with small interfering RNAs (siRNAs), and this particle is present in a soluble form, as well as associated with polyribosomes. A genetic knockout of AGO1 leads to a loss of RNAi, and concomitantly, endogenous retroposon-derived siRNAs as well as siRNAs derived from transgenic double-stranded RNA are reduced to almost undetectable levels. Furthermore, AGO1 deficiency leads to an increase in retroposon transcript abundance via mechanisms operating at the transcriptional level and at the RNA stability level. Our results suggest that AGO1 function is required for production and/or stabilization of siRNAs and provide the first evidence for an Argonaute protein being involved in the regulation of retroposon transcript levels.
机译：Argonaute蛋白家族的成员已通过遗传和生化研究的组合与RNA干扰（RNAi）和相关现象联系在一起。在这里，我们描述了 Trypanosoma brucei 中的第一个Argonaute蛋白（AGO1）的特征，这是迄今为止描述RNAi的最早的真核生物。 AGO1主要是细胞质，存在于具有小干扰RNA（siRNA）的核糖核蛋白颗粒中，该颗粒以可溶形式存在，并与多核糖体相关。 AGO1 的基因敲除导致RNAi的丧失，随之而来的是内源性逆转录子来源的siRNA以及源自转基因双链RNA的siRNA降低到几乎不可检测的水平。此外，AGO1缺乏症通过在转录水平和RNA稳定水平起作用的机制导致反转录子转录本丰度的增加。我们的结果表明，AGO1功能是生产和/或稳定siRNA所必需的，并为Argonaute蛋白参与反转录子转录水平的调控提供了第一个证据。

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