The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells

Heidi L. Cook; Hannah E. Mischo; Joan A. Steitz

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The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells

机译：疱疹病毒saimiri小核RNA募集富含AU的元素结合蛋白，但不会改变病毒转化T细胞中含有宿主富含AU的元素的mRNA水平

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Herpesvirus saimiri (HVS) encodes seven Sm-class small nuclear RNAs, called HSURs (for Herpesvirus saimiri U RNAs), that are abundantly expressed in HVS-transformed, latently infected marmoset T cells but are of unknown function. HSURs 1, 2, and 5 have highly conserved 5′-end sequences containing the AUUUA pentamer characteristic of AU-rich elements (AREs) that regulate the stability of many host mRNAs, including those encoding most proto-oncogenes and cytokines. To test whether the ARE-containing HSURs act to sequester host proteins that regulate the decay of these mRNAs, we demonstrate their in vivo interaction with the ARE-binding proteins hnRNP D and HuR in HVS-transformed T cells using a new cross-linking assay. Comprehensive Northern and microarray analyses revealed, however, that the levels of endogenous ARE-containing mRNAs are not altered in T cells latently infected with HVS mutants lacking HSURs 1 and 2. HSUR 1 binds the destabilizing ARE-binding protein tristetraprolin induced following activation of HVS-transformed T cells, but even in such stimulated cells, the levels of host ARE-containing mRNAs are not altered by deletion of HSURs 1 and 2. Instead, HSUR 1 itself is degraded by an ARE-dependent pathway in HVS-transformed T cells, suggesting that HVS may take advantage of the host ARE-mediated mRNA decay pathway to regulate HSUR expression. This is the first example of posttranscriptional regulation of the expression of an Sm small nuclear RNA.

机译： Herpesvirus saimiri （HVS）编码7个Sm级小核RNA，称为HSURs（用于 Herpesvirus saimiri U RNA），它们在HVS转化的潜伏感染的猿猴中大量表达T细胞但功能未知。 HSUR 1、2和5具有高度保守的5'端序列，其中包含AUUUA五聚体（富含AU的元件）的特征，该元件调节许多宿主mRNA的稳定性，包括编码大多数原癌基因和细胞因子的那些mRNA。为了测试含ARE的HSUR是否能螯合调节这些mRNA衰变的宿主蛋白，我们使用新的交联实验证明了它们与ARE结合蛋白hnRNP D和HuR在HVS转化的T细胞中的体内相互作用。全面的Northern和微阵列分析表明，在潜伏感染缺乏HSURs 1和2的HVS突变体的T细胞中，内源性含ARE的mRNA的水平没有改变。HSUR1与激活HVS后诱导的去稳定化的ARE结合蛋白tristetraprolin结合。转化的T细胞，但即使在这种刺激的细胞中，包含宿主ARE的mRNA的水平也不会因HSURs 1和2的缺失而改变。相反，HSUR 1本身在HVS转化的T细胞中被ARE依赖性途径降解提示HVS可能利用宿主ARE介导的mRNA衰变途径来调节HSUR表达。这是转录后调控Sm小核RNA表达的第一个例子。 展开▼

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《Molecular and Cellular Biology》 |2004年第10期|共12页

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Heidi L. Cook; Hannah E. Mischo; Joan A. Steitz;
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中图分类细胞生物学;

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1. Tristetraprolin Recruits Eukaryotic Initiation Factor 4E2 To Repress Translation of AU-Rich Element-Containing mRNAs [J] . Xianzun Tao, Guangxia Gao Molecular and Cellular Biology . 2015,第22期

机译：Tristetraprolin募集真核起始因子4E2抑制富含AU的mRNA的翻译。

2. Early Recruitment of AU-Rich Element-Containing mRNAs Determines Their Cytosolic Fate during Iron Deficiency [J] . Sandra V. Vergara, Sergi Puig, Dennis J. Thiele Molecular and Cellular Biology . 2011,第3期

机译：富含AU的mRNA的早期招募决定了铁缺乏时它们的胞质命运

3. Oncogenic Ras and transforming growth factor-beta synergistically regulate AU-rich element-containing mRNAs during epithelial to mesenchymal transition. [J] . Kanies CL, Smith JJ, Kis C, Molecular cancer research: MCR . 2008,第7期

机译：致癌性Ras和转化生长因子-β在上皮到间充质过渡过程中协同调节富含AU的含元素mRNA。

4. Application of stable isotope-labeled protein fragments to investigate the correlation of protein and mRNA levels in human cell lines [C] . Tove Bostrom, Frida Danielsson, Emma Lundberg, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：稳定同位素标记的蛋白片段的应用研究人细胞系中蛋白质和mRNA水平的相关性

5. The HSV-1 regulatory protein ICP27 interacts with key host cell proteins to mediate the inhibition of pre-mRNA splicing and export of intronless viral mRNA. [D] . Sciabica, Kathryn Sullivan. 2001

机译：HSV-1调节蛋白ICP27与关键宿主细胞蛋白相互作用，以介导抑制前mRNA剪接和无内含子病毒mRNA的输出。

6. The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells [O] . Heidi L. Cook, Hannah E. Mischo, Joan A. Steitz 2004

机译：疱疹病毒saimiri小核RNA募集富含AU的元素结合蛋白但不会改变病毒转化T细胞中含有宿主富含AU的元素的mRNA水平

7. The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells [O] . Cook, Heidi L., Mischo, Hannah E., Steitz, Joan A. 2004

机译：疱疹病毒saimiri小核RNA募集富含AU的元素结合蛋白，但不会改变病毒转化T细胞中含有宿主富含AU的元素的mRNA水平

8. Homologous Up-Regulation of the Gonadotropin-Releasing Hormone Receptor inalphaT3-1 Cells Is Associated with Unchanged Receptor Messenger RNA (mRNA) Levels and Altered mRNA Activity [R] . Tsutsumi, M., Laws, S. C., Sealfon, S. C. 1993

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机译：分离的负义单链RNA病毒，分离的或重组的核酸或其特定功能的mpv片段，载体，宿主细胞，分离的或重组的蛋白质分子或其特定的功能mpv片段，抗原，抗体，将病毒分离物鉴定为mpv，病毒分离物，用于病毒学和血清学诊断哺乳动物mpv感染的方法，用于诊断mpv感染的诊断试剂盒，病毒，核酸，载体，细胞宿主，蛋白质分子或其片段，抗原的用途，或抗体，药物组合物，用于治疗或预防mpv感染和呼吸道疾病以及获得可用于治疗疾病的抗病毒剂的方法。呼吸道，抗病毒药，病毒药的使用，禽apv感染的病毒学诊断和逻辑诊断方法，诊断测试的使用以及检测样品中针对mpv的抗体的方法

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The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells

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