Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression

Michael Pierce; Kirsten R. Benjamin; Sherwin P. Montano; Millie M. Georgiadis; Edward Winter; Andrew K. Vershon

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Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression

机译：Sum1和Ndt80蛋白质竞争绑定到控制减数分裂基因表达的中间孢子形成元素序列。

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A key transition in meiosis is the exit from prophase and entry into the nuclear divisions, which in the yeast Saccharomyces cerevisiae depends upon induction of the middle sporulation genes. Ndt80 is the primary transcriptional activator of the middle sporulation genes and binds to a DNA sequence element termed the middle sporulation element (MSE). Sum1 is a transcriptional repressor that binds to MSEs and represses middle sporulation genes during mitosis and early sporulation. We demonstrate that Sum1 and Ndt80 have overlapping yet distinct sequence requirements for binding to and acting at variant MSEs. Whole-genome expression analysis identified a subset of middle sporulation genes that was derepressed in a sum1 mutant. A comparison of the MSEs in the Sum1-repressible promoters and MSEs from other middle sporulation genes revealed that there are distinct classes of MSEs. We show that Sum1 and Ndt80 compete for binding to MSEs and that small changes in the sequence of an MSE can yield large differences in which protein is bound. Our results provide a mechanism for differentially regulating the expression of middle sporulation genes through the competition between the Sum1 repressor and the Ndt80 activator.

机译：减数分裂的关键转变是前期的退出和核分裂的进入，在酵母中，酿酒酵母取决于中间孢子形成基因的诱导。 Ndt80是中间孢子形成基因的主要转录激活因子，与称为中间孢子形成元件（MSE）的DNA序列元件结合。 Sum1是一种转录抑制因子，可与MSE结合并在有丝分裂和早期孢子形成过程中抑制中间孢子形成基因。我们证明，Sum1和Ndt80具有重叠但截然不同的序列要求，以与变体MSE结合并起作用。全基因组表达分析确定了在 sum1 突变体中被抑制的中间孢子形成基因的一个子集。 Sum1阻遏型启动子中的MSE与来自其他中间孢子形成基因的MSE的比较表明，MSE存在不同的类别。我们显示，Sum1和Ndt80竞争与MSE的结合，MSE序列的细微变化可产生结合蛋白质的较大差异。我们的结果提供了一种机制，可通过Sum1阻遏物和Ndt80激活子之间的竞争来差异调节中间孢子形成基因的表达。 展开▼

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《Molecular and Cellular Biology》 |2003年第14期|共12页

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Michael Pierce; Kirsten R. Benjamin; Sherwin P. Montano; Millie M. Georgiadis; Edward Winter; Andrew K. Vershon;
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中图分类细胞生物学;

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1. NDT80 and the Meiotic Recombination Checkpoint Regulate Expression of Middle Sporulation-Specific Genes in Saccharomyces cerevisiae [J] . Shelley R. Hepworth, Helena Friesen, Jacqueline Segall Molecular and Cellular Biology . 1998,第10期

机译：NDT80和减数分裂重组检查点调节酿酒酵母中特定孢子基因的表达。

2. Early endodermal expression of the Xenopus Endodermin gene is driven by regulatory sequences containing essential Sox protein-binding elements. [J] . Ahmed N, Howard L, Woodland HR Differentiation: The Journal of the International Society of Differentiation . 2004,第4期

机译：Xenopus Endodermin基因的早期内胚层表达受包含必需的Sox蛋白结合元件的调控序列驱动。

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机译：Sum1阻遏物在酿酒酵母中对中间减数分裂基因表达的调节。

6. Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression [O] . Michael Pierce, Kirsten R. Benjamin, Sherwin P. Montano, 2003

机译：Sum1和Ndt80蛋白竞争绑定到控制减数分裂基因表达的中间孢子形成元素序列。

7. Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression [O] . Pierce, Michael, Benjamin, Kirsten R., Montano, Sherwin P., 2003

机译：Sum1和Ndt80蛋白竞争绑定到控制减数分裂基因表达的中间孢子形成元素序列。

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机译：巨大芽孢杆菌小，酸可溶性孢子蛋白的基因：两个基因的核苷酸序列及其在孢子形成过程中的表达

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Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression

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