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首页> 外文期刊>Molecular and Cellular Biology >Cooperative activation of transcription by bovine papillomavirus type 1 E2 can occur over a large distance.
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Cooperative activation of transcription by bovine papillomavirus type 1 E2 can occur over a large distance.

机译：牛乳头瘤病毒1型E2协同激活转录可能会发生很长一段距离。

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The viral transcriptional factors encoded by the E2 open reading frame bind to the specific DNA sequence elements ACCGNNNNCGGT, allowing activation or repression of transcription. We have analyzed bovine papillomavirus type 1 E2 transactivation using recombinant genes containing E2-binding sites inserted at either 3' or 5' positions relative to the heterologous transcriptional initiation site of the herpes simplex virus thymidine kinase gene. In these hybrid plasmids, strong transactivation required the presence of a minimum of two E2-binding sites in close proximity to the promoter or five binding sites at a distance. The presence of a single E2-binding motif 5', close to the initiation site, increased the efficiency of E2 transactivation from a distance in a more-than-additive manner. Since each E2-binding site bound a dimer of the E2 protein, these experiments suggest that transactivation by E2 requires the interaction between several E2 dimers with other essential transcription factors. This interaction may be facilitated by DNA looping, which would bring E2 molecules close to the promoter.
机译：由E2开放阅读框编码的病毒转录因子与特定的DNA序列元件ACCGNNNNCGGT结合，从而可以激活或抑制转录。我们已经使用重组基因分析了牛乳头瘤病毒1型E2反式激活，该重组基因包含在相对于单纯疱疹病毒胸苷激酶基因的异源转录起始位点3'或5'位置插入的E2结合位点。在这些杂种质粒中，强反式激活要求在启动子附近至少存在两个E2结合位点，或者在一定距离处存在五个结合位点。靠近起始位点的单个E2结合基序5'的存在以远大于加性的方式从远处提高了E2反式激活的效率。由于每个E2结合位点都结合了E2蛋白的二聚体，因此这些实验表明，通过E2进行反式激活需要几个E2二聚体与其他必需转录因子之间的相互作用。 DNA环化可促进这种相互作用，这将使E2分子靠近启动子。

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