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首页> 外文期刊>Molecular and Cellular Biology >The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose.
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The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose.

机译：酵母UME5基因调节葡萄糖减数分裂mRNA的稳定性。

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We reported previously that early meiotic transcripts are highly unstable. These mRNAs exhibit half-lives of approximately 3 min when expressed during vegetative growth in glucose medium and are stabilized twofold during sporulation in acetate medium. Two genes, UME2 and UME5, that regulate the stability of meiosis-specific transcripts have been identified. The wild-type UME5 gene, which has been analyzed in detail, decreases the stability of all meiotic mRNAs tested approximately twofold when expressed during vegetative growth but has no effect on the half-lives of a number of vegetative mRNAs examined. The UME5 gene is dispensable for mitotic and meiotic development. Cells in which the entire UME5 gene has been deleted are viable, although the generation time is slightly longer and sporulation is less efficient. The UME5 transcript is constitutively expressed, and its stability is not autoregulated. The UME5 gene encodes a predicted 63-kDa protein with homology to the family of CDC28 serine/threonine-specific protein kinases. The kinase activity appears to be central to the function of the UME5 protein, since alteration of a highly conserved amino acid in the kinase domain results in a phenotype identical to that of a ume5 deletion. Genetic epistasis studies suggest that the UME2 and UME5 gene products act in the same pathway to regulate meiotic transcript stability. This pathway is independent of deadenylation and translation, two factors known to be important in regulating mRNA turnover. Significantly, the UME5-mediated destabilization of meiotic mRNAs occurs in glucose- but not in acetate-containing medium. Thus, the UME5 gene appears to participate in a glucose signal transduction pathway governing message stability.
机译：我们以前曾报道过，早期减数分裂转录本非常不稳定。当在葡萄糖培养基中营养生长期间表达时，这些mRNA的半衰期约为3分钟，而在醋酸盐培养基中形成孢子时则稳定两倍。已经确定了调节减数分裂特异性转录物稳定性的两个基因，UME2和UME5。经过详细分析的野生型UME5基因，在营养生长过程中表达时，降低了测试的所有减数分裂mRNA的稳定性，大约是原来的两倍，但对许多被检测的营养mRNA的半衰期没有影响。 UME5基因对于有丝分裂和减数分裂发育是必不可少的。完整的UME5基因已被删除的细胞是可行的，尽管生成时间略长且孢子形成效率较低。 UME5转录本组成型表达，其稳定性不是自动调节的。 UME5基因编码与CDC28丝氨酸/苏氨酸特异性蛋白激酶家族同源的预测的63 kDa蛋白。激酶活性似乎是UME5蛋白功能的核心，因为激酶结构域中高度保守的氨基酸的改变会导致表型与ume5缺失相同。遗传上位研究表明，UME2和UME5基因产物以相同的途径调节减数分裂转录物的稳定性。该途径不依赖于腺苷酸化和翻译，这是已知在调节mRNA更新中重要的两个因素。值得注意的是，UME5介导的减数分裂mRNA失稳发生在含葡萄糖的培养基中，而不发生在含乙酸盐的培养基中。因此，UME5基因似乎参与了控制消息稳定性的葡萄糖信号转导途径。

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