...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.
【24h】

Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.

机译：发育中的心肌细胞增强因子2转录因子家族的差异表达：心脏因子BBF-1是心脏发生的早期标记。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the present study, we have used single chicken blastoderms of defined early developmental stages, beginning with the prestreak stage, stage 1 (V. Hamburger and H. L. Hamilton, J. Morphol. 88:49-92, 1951), to analyze the onset of cardiac myogenesis by monitoring the appearance of selected cardiac muscle tissue-specific gene transcripts and the functional expression of the myocyte enhancer factor 2 (MEF-2) proteins. Using gene-specific oligonucleotide primers in reverse transcriptase PCR assay, we have demonstrated that the cardiac myosin light-chain 2 (MLC2) and alpha-actin gene transcripts appear as early as stage 5, i.e., immediately after the cardiogenic fate assignment at stage 4. Consistent with this observation is the developmental expression pattern of DNA-binding activity of BBF-1, a cardiac muscle-specific member of the MEF-2 protein family, which also begins at stage 5 prior to MEF-2. Differential expression of DNA-binding complexes is also observed with another AT-rich DNA sequence (CArG box) as probe, but the binding pattern with the ubiquitous TATA-binding proteins remains unchanged during the same developmental period. Thus, the cardiogenic commitment and differentiation of the precardiac mesoderm, as exemplified by the appearance of cardiac MEF-2, MLC2, and alpha-actin gene products, occur earlier than previously thought and appear to be closely linked. The onset of skeletal myogenic program follows that of the cardiogenic program with the appearance of skeletal MLC2 at stage 8. We also observed that mRNA for the MEF-2 family of proteins appears as early as stage 2 and that for CMD-1, the chicken counterpart of MyoD, appears at stage 5. The temporal separation of activation of cardiac and skeletal MLC2 genes, which appears immediately after the respective fate assignments, and those of cardiac MEF-2 and CMD-1, which occur before, are consistent with the established appearance of the myogenic programs and with the acquisition pattern of the two tissue-specific morphological characteristics in the early embryo. The preferential appearance of BBF-1 activity in precardiac moesderm, relative to that of MEF-2, indicates that these two protein factors are distinct members of the MEF-2 family and provides a compelling argument in support of the potential role of BBF-1 as a regulator of the cardiogenic cell lineage determination, while cardiac MEF-2 might be involved in maintenance of the cardiac differentiative state.
机译：在本研究中，我们使用了已定义的早期发育阶段的单个鸡胚盘，从前期阶段开始，即第1阶段（V. Hamburger和HL Hamilton，J。Morphol。88：49-92，1951年）来分析发病情况。通过监测选定的心肌组织特异性基因转录本的出现以及心肌细胞增强因子2（MEF-2）蛋白的功能性表达来监测心肌的发生。使用基因特异性寡核苷酸引物进行逆转录酶PCR检测，我们证明了心肌肌球蛋白轻链2（MLC2）和α-肌动蛋白基因转录本最早出现在第5阶段，即在第4阶段心源性命运确定后与该观察结果一致的是，BBF-1（一种MEF-2蛋白家族的心肌特异性成员）的DNA结合活性的发育表达模式，也始于MEF-2的第5阶段。在另一个富含AT的DNA序列（CArG盒）作为探针的情况下，也观察到了DNA结合复合物的差异表达，但是在相同的发育时期，与普遍存在的TATA结合蛋白的结合模式保持不变。因此，以心脏MEF-2，MLC2和α-肌动蛋白基因产物的出现为例，心前中胚层的心源性承诺和分化早于以前的想象，并且似乎密切相关。骨骼肌成肌程序的发作跟心源性程序的发作一样，在阶段8出现骨骼MLC2。我们还观察到，MEF-2蛋白家族的mRNA最早出现在2期，而鸡CMD-1的mRNA出现。 MyoD的对应物出现在第5阶段。心脏和骨骼MLC2基因激活的时间间隔（分别出现在各自的命运分配之后，以及之前出现的心脏MEF-2和CMD-1的时间间隔）与建立了肌源性程序的外观，并在早期胚胎中获得了两种组织特异性形态特征的获取模式。相对于MEF-2而言，心前皮中BBF-1活性的优先出现表明这两个蛋白因子是MEF-2家族的不同成员，并提供了令人信服的论点来支持BBF-1的潜在作用作为心脏细胞谱系确定的调节剂，而心脏MEF-2可能参与了心脏分化状态的维持。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号