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首页> 外文期刊>Molecular and Cellular Biology >ELF-2, a new member of the Eph ligand family, is segmentally expressed in mouse embryos in the region of the hindbrain and newly forming somites.
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ELF-2, a new member of the Eph ligand family, is segmentally expressed in mouse embryos in the region of the hindbrain and newly forming somites.

机译：Eph配体家族的新成员ELF-2在小鼠胚胎的后脑和新形成的节节区域中分段表达。

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The Eph receptors are the largest known family of receptor tyrosine kinases and are notable for distinctive expression patterns in the nervous system and in early vertebrate development. However, all were identified as orphan receptors, and only recently have there been descriptions of a corresponding family of ligands. We describe here a new member of the Eph ligand family, designated ELF-2 (Eph ligand family 2). The cDNA sequence for mouse ELF-2 indicates that it is a transmembrane ligand. It shows closest homology to the other known transmembrane ligand in the family, ELK-L/LERK-2/Cek5-L, with 57% identity in the extracellular domain. There is also striking homology in the cytoplasmic domain, including complete identity of the last 33 amino acids, suggesting intracellular interactions. On cell surfaces, and in a cell-free system, ELF-2 binds to three closely related Eph family receptors, Elk, Cek10 (apparent ortholog of Sek-4 and HEK2), and Cek5 (apparent ortholog of Nuk/Sek-3), all with dissociation constants of approximately 1 nM. In situ hybridization of mouse embryos shows ELF-2 RNA expression in a segmental pattern in the hindbrain region and the segmenting mesoderm. Comparable patterns have been described for Eph family receptors, including Sek-4 and Nuk/Sek-3, suggesting roles for ELF-2 in patterning these regions of the embryo.
机译：Eph受体是受体酪氨酸激酶的最大已知家族，并在神经系统和早期脊椎动物发育中表现出独特的表达模式。然而，所有这些都被鉴定为孤儿受体，直到最近才有对相应配体家族的描述。我们在这里描述了Eph配体家族的一个新成员，称为ELF-2（Eph配体家族2）。小鼠ELF-2的cDNA序列表明它是跨膜配体。它显示出与家族中其他已知跨膜配体ELK-L / LERK-2 / Cek5-L的最接近同源性，在细胞外结构域具有57％的同一性。在细胞质结构域中也存在惊人的同源性，包括最后33个氨基酸的完全同一性，表明细胞内相互作用。在细胞表面和无细胞系统中，ELF-2与三个密切相关的Eph家族受体Elk，Cek10（Sek-4和HEK2的表观同源物）和Cek5（Nuk / Sek-3的表观同源物）结合。 ，所有的解离常数约为1 nM。小鼠胚胎的原位杂交显示ELF-2 RNA在后脑区域和中胚层中呈分段模式表达。已经描述了包括Sek-4和Nuk / Sek-3在内的Eph家族受体的可比模式，表明ELF-2在使胚胎的这些区域图案化中的作用。

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