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首页> 外文期刊>Molecular reproduction and development >FGF ligand family mRNA expression profile for mouse preimplantation embryos, early gestation human placenta, and mouse trophoblast stem cells
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FGF ligand family mRNA expression profile for mouse preimplantation embryos, early gestation human placenta, and mouse trophoblast stem cells

机译：小鼠植入前胚胎，早期妊娠人胎盘和小鼠滋养层干细胞的FGF配体家族mRNA表达谱

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Signaling by fibroblast growth factor (FGF) is essential is for trophoblast stem (TS) cells and preimplantation embryos. FGF4 provides essential signaling, but the expression of the complete set of 23 FGF family members has not been analyzed. Here, semi-quantitative RT-PCR and microarray analyses were used to define expression of all FGF ligand mRNA. RT-PCR was done for developmentally important FGF subfamilies, FGF10/FGF22 and FGF8/FGF17/FGF18 as well as FGF11. FGF4 and FGF18 are detected at highest levels by RT-PCR and microarrays. FGF10 was detected at low levels in both assays. FGF11 was detected at moderate levels by microarray, but not by RT-PCR. FGF17 was detected at low levels by array and moderate levels by RT-PCR. FGF8 and FGF22 were detected by RT-PCR, but not by microarrays during late cleavage divisions. FGF8, FGF5, and FGF9 were detected in the oocyte by microarray. FGF2, FGF3, and FGF7 were not detected by RT-PCR or microarrays and FGF13, FGF14, and FGF23 were not detected by microarray. Since a major role of FGF is to maintain TS cells, we tested human and mouse placental cell lines and early gestation human placenta for expression of FGF ligands. Expression in mouse TS cells was compared with preimplantation embryos, and human placental cell line expression was compared with human placenta, to infer which ligands are expressed in placental lineage vs. other cell lineages. The data suggest that human and mouse placenta share FGF18 and its high expression suggests preimplantation and early placental function.
机译：成纤维细胞生长因子（FGF）发出的信号对于滋养层干（TS）细胞和植入前胚胎至关重要。 FGF4提供必需的信号传导，但尚未分析23个FGF家族成员的完整表达。在这里，使用半定量RT-PCR和微阵列分析来定义所有FGF配体mRNA的表达。对发育重要的FGF亚家族FGF10 / FGF22和FGF8 / FGF17 / FGF18以及FGF11进行了RT-PCR。通过RT-PCR和微阵列检测到最高水平的FGF4和FGF18。在两种测定中均检测到低水平的FGF10。通过微阵列检测到了中等水平的FGF11，但是通过RT-PCR检测不到。阵列检测到的FGF17水平较低，RT-PCR检测到的水平中等水平。通过RT-PCR检测到FGF8和FGF22，但是在后期切割分裂期间未通过微阵列检测到。通过微阵列在卵母细胞中检测到FGF8，FGF5和FGF9。 RT-PCR或微阵列未检测到FGF2，FGF3和FGF7，微阵列未检测到FGF13，FGF14和FGF23。由于FGF的主要作用是维持TS细胞，因此我们测试了人类和小鼠胎盘细胞系以及早期妊娠的人类胎盘中FGF配体的表达。将小鼠TS细胞中的表达与植入前的胚胎进行比较，并将人胎盘细胞系的表达与人胎盘进行比较，以推断哪些配体在胎盘谱系与其他细胞谱系中表达。数据表明人胎盘和小鼠胎盘共享FGF18，其高表达提示着植入前和胎盘早期功能。

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