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首页> 外文期刊>Molecular and Cellular Biology >A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein.
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A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein.

机译：在睾丸中表达的与SRY相关的基因编码含有亮氨酸拉链的蛋白质。

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SRY-related cDNA encoding a protein with a high-mobility-group (HMG) box and a leucine zipper motif, which was designated SOX-LZ, was isolated from a rainbow trout testis cDNA library. Comparison of this cDNA with the mouse homologous cDNA isolated from a testis cDNA library exhibits an overall amino acid sequence identity of 77%, which is in striking contrast to the abrupt loss of amino acid sequence homology outside the HMG box found among mammalian SRY genes. In both rainbow trout and mice, Northern (RNA) blot analyses have revealed the presence of a testis-specific 3-kb-long SOX-LZ mRNA, and this transcript appeared coincidentally with the protamine mRNA, suggesting its expression in the germ line. A recombinant HMG box region protein encoded by SOX-LZ could bind strongly with an oligonucleotide containing an AACAAT sequence, which is also recognized by mouse Sry and Sox-5. Upon cotransfection into CHO cells, SOX-LZ transactivated transcription through its binding motif when the region including the leucine zipper motif was deleted [SOX-LZ (D105-356)]; however, the intact SOX-LZ failed to transactivate. The intact SOX-LZ could form homodimers through the leucine zipper, which resulted in inhibition of DNA binding by the HMG box, while SOX-LZ (D105-356), which was incapable of dimerization, showed specific binding with the AACAAT sequence. Thus, the repressed transactivation of the intact SOX-LZ in CHO cells was primarily attributable to the low level of DNA binding of SOX-LZ homodimers.
机译：从虹鳟鱼睾丸cDNA文库中分离出编码具有高迁移率基团（HMG）框和亮氨酸拉链基序的蛋白的SRY相关cDNA，命名为SOX-LZ。该cDNA与从睾丸cDNA文库中分离的小鼠同源cDNA的比较显示出77％的总氨基酸序列同一性，这与在哺乳动物SRY基因中发现的HMG盒外的氨基酸序列同源性突然丧失形成鲜明对比。在虹鳟鱼和小鼠中，Northern（RNA）印迹分析表明存在睾丸特异性3kb长的SOX-LZ mRNA，并且该转录本与鱼精蛋白mRNA一致出现，表明其在种系中表达。由SOX-LZ编码的重组HMG盒区域蛋白可以与包含AACAAT序列的寡核苷酸牢固结合，该序列也被小鼠Sry和Sox-5识别。当共转染到CHO细胞中时，当删除了包括亮氨酸拉链基序的区域时，SOX-LZ通过其结合基序使转录激活[SOX-LZ（D105-356）]。但是，完整的SOX-LZ无法激活。完整的SOX-LZ可以通过亮氨酸拉链形成同型二聚体，从而导致HMG盒抑制DNA结合，而不能二聚化的SOX-LZ（D105-356）显示出与AACAAT序列的特异性结合。因此，完整的SOX-LZ在CHO细胞中受阻的反式激活主要归因于SOX-LZ同二聚体的低水平DNA结合。

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