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首页> 外文期刊>Molecular and Cellular Biology >A Role for Groucho Tetramerization in Transcriptional Repression
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A Role for Groucho Tetramerization in Transcriptional Repression

机译：Groucho四聚体在转录抑制中的作用。

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The Drosophila Groucho (Gro) protein is a corepressor required by a number of DNA-binding transcriptional repressors. Comparison of Gro with its homologues in other eukaryotic organisms reveals that Gro contains, in addition to a conserved C-terminal WD repeat domain, a conserved N-terminal domain, which has previously been implicated in transcriptional repression. We determined, via a variety of hydrodynamic measurements as well as protein cross-linking, that native Gro is a tetramer in solution and that tetramerization is mediated by two putative amphipathic α-helices (termed leucine zipper-like motifs) found in the N-terminal region. Point mutations in the leucine zipper-like motifs that block tetramerization also block repression by Gro, as assayed in cultured Drosophila cells with Gal4-Gro fusion proteins. Furthermore, the heterologous tetramerization domain from p53 fully substitutes for the Gro tetramerization domain in transcriptional repression. These findings suggest that oligomerization is essential for Gro-mediated repression and that the primary function of the conserved N-terminal domain is to mediate this oligomerization.
机译：果蝇Grocho（Gro）蛋白是许多DNA结合转录阻遏物所需要的一种核心抑制剂。将Gro及其同源物与其他真核生物进行比较后发现，Gro除包含保守的C末端WD重复结构域外，还包含一个保守的N末端结构域，该结构先前与转录抑制有关。我们通过各种流体力学测量以及蛋白质交联确定，天然Gro是溶液中的四聚体，并且四聚是由N-中发现的两个假定的两亲性α螺旋（称为亮氨酸拉链样基序）介导的。终端区域。在带有Gal4-Gro融合蛋白的 Drosophila 细胞中检测到，亮氨酸拉链样基序中的点突变可阻止四聚化，也可阻止Gro抑制。此外，来自p53的异源四聚结构域在转录抑制中完全替代了Gro四聚结构域。这些发现表明，寡聚化对于Gro介导的阻遏是必不可少的，并且保守的N末端结构域的主要功能是介导这种寡聚化。

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