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首页> 外文期刊>Molecular and Cellular Biology >The Small GTP-Binding Protein Rho Potentiates AP-1 Transcription in T Cells
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The Small GTP-Binding Protein Rho Potentiates AP-1 Transcription in T Cells

机译：小GTP结合蛋白Rho增强T细胞中的AP-1转录。

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The Rho family of small GTP-binding proteins is involved in the regulation of cytoskeletal structure, gene transcription, specific cell fate development, and transformation. We demonstrate in this report that overexpression of an activated form of Rho enhances AP-1 activity in Jurkat T cells in the presence of phorbol myristate acetate (PMA), but activated Rho (V14Rho) has little or no effect on NFAT, Oct-1, and NF-κB enhancer element activities under similar conditions. Overexpression of a V14Rho construct incapable of membrane localization (CAAX deleted) abolishes PMA-induced AP-1 transcriptional activation. The effect of Rho on AP-1 is independent of the mitogen-activated protein kinase pathway, as a dominant-negative MEK and a MEK inhibitor (PD98059) did not affect Rho-induced AP-1 activity. V14Rho binds strongly to protein kinase Cα (PKCα) in vivo; however, deletion of the CAAX site on V14Rho severely diminished this association. Evidence for a role for PKCα as an effector of Rho was obtained by the observation that coexpression of the N-terminal domain of PKCα blocked the effects of activated Rho plus PMA on AP-1 transcriptional activity. These data suggest that Rho potentiates AP-1 transcription during T-cell activation.
机译：小GTP结合蛋白的Rho家族参与细胞骨架结构，基因转录，特定细胞命运发展和转化的调控。我们在此报告中证明，在佛波醇肉豆蔻酸酯乙酸酯（PMA）存在的情况下，活化形式的Rho的过表达增强Jurkat T细胞中的AP-1活性，但活化的Rho（V14Rho）对NFAT，Oct-1几乎没有影响和NF-κB增强子在相似条件下的活性。无法膜定位（CAAX缺失）的V14Rho构建体的过表达消除了PMA诱导的AP-1转录激活。 Rho对AP-1的作用独立于丝裂原激活的蛋白激酶途径，因为显性阴性的MEK和MEK抑制剂（PD98059）不会影响Rho诱导的AP-1活性。 V14Rho在体内与蛋白激酶Cα（PKCα）牢固结合;但是，删除V14Rho上的CAAX位点会严重削弱这种关联。通过观察到PKCαN末端结构域的共表达会阻止活化的Rho加PMA对AP-1转录活性的影响，从而获得了PKCα作为Rho的效应物的证据。这些数据表明，Rho在T细胞活化过程中增强了AP-1的转录。

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