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Complex Formation of Yeast Rev1 with DNA Polymerase η

机译：酵母Rev1与DNA聚合酶η的复合物形成

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In Saccharomyces cerevisiae, Rev1 functions in translesion DNA synthesis (TLS) together with polymerase ζ (Polζ), comprised of the Rev3 catalytic and Rev7 accessory subunits. Rev1 plays an indispensable structural role in promoting Polζ function, and deletion of the Rev1-C terminal region that is involved in physical interactions with Rev3 inactivates Polζ function in TLS. In humans, however, Rev1 has been shown to physically interact with the Y-family polymerases Polη, Polι, and Polκ, and the Rev1 C terminus mediates these interactions. Since all the available genetic and biochemical evidence in yeast support the requirement of Rev1 as a structural element for Polζ and not for Polη, these observations have raised the possibility that in its structural role, Rev1 has diverged between yeast and humans. Here we show that although in yeast a stable Rev1-Polη complex can be formed, this complex formation involves the polymerase-associated domain of Rev1 and not the Rev1 C terminus as in humans. We also found that the DNA synthesis activity of Rev1 is enhanced in this complex. We discuss the implications of these and other observations for the possible divergence of Rev1's structural role between yeast and humans.
机译：在酿酒酵母中，Rev1与包含Rev3催化亚基和Rev7辅助亚基的聚合酶ζ（Polζ）一起在病灶DNA合成（TLS）中起作用。 Rev1在促进Polζ功能中起着必不可少的结构性作用，而与Rev3物理相互作用所涉及的Rev1-C末端区域的缺失则会使TLS中的Polζ功能失活。然而，在人类中，Rev1已显示与Y家族聚合酶Polη，PolI和Polκ发生物理相互作用，而Rev1 C末端介导这些相互作用。由于酵母中所有可用的遗传和生化证据均支持Rev1作为Polζ而非Polη的结构元素的要求，因此这些观察结果提出了Rev1在酵母和人类之间发散的可能性。在这里，我们表明尽管可以在酵母中形成稳定的Rev1-Polη复合物，但这种复合物的形成涉及Rev1的聚合酶相关域，而不是人类中的Rev1 C末端。我们还发现，在这种复合物中，Rev1的DNA合成活性得到增强。我们讨论这些和其他观察结果对酵母和人之间Rev1的结构作用可能存在的分歧的影响。

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