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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Yeast Rev1 protein promotes complex formation of DNA polymerase ζ with Pol32 subunit of DNA polymerase δ
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Yeast Rev1 protein promotes complex formation of DNA polymerase ζ with Pol32 subunit of DNA polymerase δ

机译：酵母Rev1蛋白促进DNA聚合酶ζ与DNA聚合酶δ的Pol32亚基的复杂形成

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摘要

Yeast DNA polymerase (Pol) δ, essential for DNA replication, is comprised of 3 subunits, Pol3, Pol31, and Pol32. Of these, the catalytic subunit Pol3 and the second subunit Pol31 are essential, whereas the Pol32 subunit is not essential for DNA replication. Although Pol32 is an integral component of Polδ, it is also required for translesion synthesis (TLS) by Polζ. To begin to decipher the bases of Pol32 involvement in Polζ-mediated TLS, here we examine whether Pol32 physically interacts with Polζ or its associated proteins and provide evidence for the physical interaction of Pol32 with Rev1. Rev1 plays an indispensable structural role in Polζ-mediated TLS and it binds the Rev3 catalytic subunit of Polζ. Here, we show that although Pol32 does not directly bind Polζ, Pol32 can bind the Rev1-Polζ complex through its interaction with Rev1. We find that Pol32 binding has no stimulatory effect on DNA synthesis either by Rev1 in the Rev1-Pol32 complex or by Polζ in the Polζ-Rev1-Pol32 complex, irrespective of whether proliferating cell nuclear antigen has been loaded onto DNA or not. We discuss evidence for the biological significance of Rev1 binding to Pol32 for Polζ function in TLS and suggest a structural role for Rev1 in modulating the binding of Polζ with Pol32 in Polδ stalled at a lesion site.
机译：酵母DNA聚合酶（Pol）δ是DNA复制所必需的，由3个亚基Pol3，Pol31和Pol32组成。其中，催化亚基Pol3和第二亚基Pol31是必需的，而Pol32亚基对于DNA复制不是必需的。尽管Pol32是Polδ不可或缺的组成部分，但它也是Polζ进行跨病变合成（TLS）所必需的。为了开始破译Pol32参与Polζ介导的TLS的基础，在这里我们检查Pol32是否与Polζ或其相关蛋白发生物理相互作用，并为Pol32与Rev1的物理相互作用提供证据。 Rev1在Polζ介导的TLS中起必不可少的结构作用，它结合了Polζ的Rev3催化亚基。在这里，我们显示尽管Pol32不直接结合Polζ，但Pol32可以通过与Rev1相互作用来结合Rev1-Polζ复合物。我们发现，无论增殖细胞核抗原是否已加载到DNA上，Pol32结合都不会通过Rev1-Pol32复合物中的Rev1或Polζ-Rev1-Pol32复合物中的Polζ对DNA合成产生刺激作用。我们讨论了Rev1结合Pol32在TLS中对Polζ功能的生物学意义的证据，并提出Rev1在调节停滞在病变部位的Polδ中与Pol32结合的Polζ的结构性作用。

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  • 作者

    Narottam Acharya; Robert E. Johnson; Vincent Pages; Louise Prakash; Satya Prakash;

  • 作者单位

    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1061;

    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1061;

    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1061;

    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1061;

    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1061;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    DNA polymerase ζ-Rev1-Pol32 complex; Rev1 interaction with Pol32; translesion synthesis;

    机译：DNA聚合酶ζ-Rev1-Pol32复合物;Rev1与Pol32的交互;病灶合成;

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