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首页> 外文期刊>Molecular and Cellular Biology >Regulation of Nuclear Positioning and Dynamics of the Silent Mating Type Loci by the Yeast Ku70/Ku80 Complex
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Regulation of Nuclear Positioning and Dynamics of the Silent Mating Type Loci by the Yeast Ku70/Ku80 Complex

机译：Ku70 / Ku80酵母复合物对沉默交配型基因座的核定位和动力学的调控。

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We have examined the hypothesis that the highly selective recombination of an active mating type locus (MAT) with either HMLα or HMR>a is facilitated by the spatial positioning of relevant sequences within the budding yeast (Saccharomyces cerevisiae) nucleus. However, both position relative to the nuclear envelope (NE) and the subnuclear mobility of fluorescently tagged MAT, HML, or HMR loci are largely identical in haploid >a and α cells. Irrespective of mating type, the expressed MAT locus is highly mobile within the nuclear lumen, while silent loci move less and are found preferentially near the NE. The perinuclear positions of HMR and HML are strongly compromised in strains lacking the Silent information regulator, Sir4. However, HMLα, unlike HMR>a and most telomeres, shows increased NE association in a strain lacking yeast Ku70 (yKu70). Intriguingly, we find that the yKu complex is associated with HML and HMR sequences in a mating-type-specific manner. Its abundance decreases at the HMLα donor locus and increases transiently at MAT>a following DSB induction. Our data suggest that mating-type-specific binding of yKu to HMLα creates a local chromatin structure competent for recombination, which cooperates with the recombination enhancer to direct donor choice for gene conversion of the MAT>a locus.
机译：我们检查了以下假设：活性交配型基因座（ MAT ）与 HML α或 HMR > a高度选择性重组通过萌芽酵母（）核内相关序列的空间定位而变得容易。但是，相对于核被膜（NE）的位置和荧光标记的 MAT ， HML 或 HMR 基因座的亚核迁移率基本相同在单倍体> a 和α细胞中。无论交配类型如何，表达的 MAT 基因座在核腔内均具有高度的移动性，而沉默基因座的移动较少，且优先位于NE附近。在缺少沉默信息调节因子Sir4的菌株中， HMR 和 HML 的核周位置受到严重损害。但是， HML α与 HMR > a 和大多数端粒不同，它在缺乏酵母Ku70（yKu70）的菌株中显示出NE相关性增加。有趣的是，我们发现yKu复合体以特定于交配类型的方式与 HML 和 HMR 序列相关。在DSB诱导后，其丰度在 HML α供体位点降低，并在 MAT > a 处瞬时升高。我们的数据表明，yKu与 HML α的交配类型特异性结合可形成具有重组能力的局部染色质结构，该结构与重组增强子协同作用，可直接选择供体以进行 MAT的基因转化 >一个位置。

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