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Maf1p, a Negative Effector of RNA Polymerase III inSaccharomyces cerevisiae

机译：Maf1p，在酿酒酵母中的RNA聚合酶III的负效应。

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Although yeast RNA polymerase III (Pol III) and the auxiliary factors TFIIIC and TFIIIB are well characterized, the mechanisms of class III gene regulation are poorly understood. Previous studies identified MAF1, a gene that affects tRNA suppressor efficiency and interacts genetically with Pol III. We show here that tRNA levels are elevated in maf1 mutant cells. In keeping with the higher levels of tRNA observed in vivo, the in vitro rate of Pol III RNA synthesis is significantly increased in maf1cell extracts. Mutations in the RPC160 gene encoding the largest subunit of Pol III which reduce tRNA levels were identified as suppressors of the maf1 growth defect. Interestingly, Maf1p is located in the nucleus and coimmunopurifies with epitope-tagged RNA Pol III. These results indicate that Maf1p acts as a negative effector of Pol III synthesis. This potential regulator of Pol III transcription is likely conserved since orthologs of Maf1p are present in other eukaryotes, including humans.
机译：尽管酵母RNA聚合酶III（Pol III）和辅助因子TFIIIC和TFIIIB具有很好的特征，但对III类基因调控的机制知之甚少。先前的研究鉴定了 MAF1 ，该基因会影响tRNA抑制子的效率并与Pol III发生基因相互作用。我们在这里显示， maf1 突变细胞中的tRNA水平升高。为了与体内观察到的较高水平的tRNA保持一致， maf1 细胞提取物中Pol III RNA的体外合成速率显着提高。编码tIII水平最大的Pol III最大亚基的 RPC160 基因突变被鉴定为 maf1 生长缺陷的抑制因子。有趣的是，Maf1p位于细胞核中，并用带表位标签的RNA Pol III进行共免疫纯化。这些结果表明，Maf1p充当Pol III合成的负效应子。由于Maf1p的直系同源物存在于其他真核生物中，包括人类，Pol III转录的这种潜在调节剂可能是保守的。

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