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Transcription Activator Interactions with Multiple SWI/SNF Subunits

机译：转录激活因子与多个SWI / SNF亚基的相互作用

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We have previously shown that the yeast SWI/SNF complex stimulates in vitro transcription from chromatin templates in an ATP-dependent manner. SWI/SNF function in this regard requires the presence of an activator with which it can interact directly, linking activator recruitment of SWI/SNF to transcriptional stimulation. In this study, we determine the SWI/SNF subunits that mediate its interaction with activators. Using a photo-cross-linking label transfer strategy, we show that the Snf5, Swi1, and Swi2/Snf2 subunits are contacted by the yeast acidic activators, Gcn4 and Hap4, in the context of the intact native SWI/SNF complex. In addition, we show that the same three subunits can interact individually with acidic activation domains, indicating that each subunit contributes to binding activators. Furthermore, mutations that reduce the activation potential of these activators also diminish its interaction with each of these SWI/SNF subunits. Thus, three distinct subunits of the SWI/SNF complex contribute to its interactions with activation domains.
机译：先前我们已经表明，酵母SWI / SNF复合体以ATP依赖的方式刺激了染色质模板的体外转录。在这方面，SWI / SNF功能需要存在可以直接相互作用的激活剂，从而将SWI / SNF的激活剂募集与转录刺激联系起来。在这项研究中，我们确定介导其与激活剂相互作用的SWI / SNF亚基。使用光交联标签转移策略，我们显示在完整的天然SWI / SNF复合体的背景下，酵母酸性激活剂Gcn4和Hap4接触了Snf5，Swi1和Swi2 / Snf2亚基。此外，我们显示相同的三个亚基可以与酸性激活域单独相互作用，表明每个亚基都有助于结合激活剂。此外，降低这些激活剂激活潜能的突变也减少了其与这些SWI / SNF亚基的相互作用。因此，SWI / SNF复合体的三个不同的亚基有助于其与激活域的相互作用。

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