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首页> 外文期刊>Molecular and Cellular Biology >Rab3D Is Not Required for Exocrine Exocytosis but for Maintenance of Normally Sized Secretory Granules
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Rab3D Is Not Required for Exocrine Exocytosis but for Maintenance of Normally Sized Secretory Granules

机译：Rab3D不需要外分泌胞吐作用，但可以维持正常大小的分泌颗粒

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Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland. To shed light on the function of Rab3D, we have generated Rab3D-deficient mice. These mice are viable and have no obvious phenotypic changes. Secretion of mast cells is normal as revealed by capacitance patch clamping. Furthermore, enzyme content and overall morphology are unchanged in pancreatic and parotid acinar cells of knockout mice. Both the exocrine pancreas and the parotid gland show normal release kinetics in response to secretagogue stimulation, suggesting that Rab3D is not involved in exocytosis. However, the size of secretory granules in both the exocrine pancreas and the parotid gland is significantly increased, with the volume being doubled. We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules.
机译：Rab3D是Rab / ypt GTPases的Rab3子家族的成员，在胰腺，肥大细胞和腮腺的分泌囊泡中的酶原颗粒上表达。为了阐明Rab3D的功能，我们生成了Rab3D缺陷小鼠。这些小鼠是活的，没有明显的表型变化。肥大细胞的分泌是正常的，如通过电容膜片钳位所揭示的。此外，基因敲除小鼠的胰腺和腮腺腺泡细胞中的酶含量和总体形态没有变化。外分泌胰腺和腮腺均显示出对促分泌素刺激的正常释放动力学，表明Rab3D不参与胞吐作用。但是，外分泌胰腺和腮腺中分泌颗粒的大小均显着增加，体积增加了一倍。我们得出结论，Rab3D可能通过防止分泌颗粒的同型融合来发挥其在颗粒成熟过程中的功能。

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