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首页> 外文期刊>Molecular and Cellular Biology >Evidence for Eviction and Rapid Deposition of Histones upon Transcriptional Elongation by RNA Polymerase II
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Evidence for Eviction and Rapid Deposition of Histones upon Transcriptional Elongation by RNA Polymerase II

机译：RNA聚合酶II转录伸长时将组蛋白逐出和快速沉积的证据

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Biochemical experiments indicate that transcriptional elongation by RNA polymerase II (Pol II) is inhibited by nucleosomes and hence requires chromatin-modifying activities. Here, we examine the fate of histones upon passage of elongating Pol II in vivo. Histone density throughout the entire Saccharomyces cerevisiae GAL10 coding region is inversely correlated with Pol II association and transcriptional activity, suggesting that the elongating Pol II machinery efficiently evicts core histones from the DNA. Furthermore, new histones appear to be deposited onto DNA less than 1 min after passage of Pol II. Transcription-dependent deposition of histones requires the FACT complex that travels with elongating Pol II. Our results suggest that Pol II transcription generates a highly dynamic equilibrium of histone eviction and histone deposition and that there is significant histone exchange throughout most of the yeast genome within a single cell cycle.
机译：生化实验表明，RNA聚合酶II（Pol II）的转录伸长被核小体抑制，因此需要染色质修饰活性。在这里，我们检查了组蛋白在体内延长Pol II通过时的命运。整个酿酒酵母GAL10 编码区中的组蛋白密度与Pol II缔合和转录活性呈负相关，这表明延伸的Pol II机制可以有效地从DNA中清除核心组蛋白。此外，新的组蛋白似乎在通过Pol II后不到1分钟的时间内沉积到了DNA上。依赖转录的组蛋白沉积需要FACT复合物，该复合物随Pol II的延长而移动。我们的结果表明，Pol II转录产生了组蛋白逐出和组蛋白沉积的高度动态平衡，并且在单个细胞周期内，整个大多数酵母基因组中都有明显的组蛋白交换。

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