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首页> 外文期刊>Molecular and Cellular Biology >Posttranscriptional regulation of rat growth hormone gene expression: increased message stability and nuclear polyadenylation accompany thyroid hormone depletion.
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Posttranscriptional regulation of rat growth hormone gene expression: increased message stability and nuclear polyadenylation accompany thyroid hormone depletion.

机译：大鼠生长激素基因表达的转录后调控：信息稳定性增强和核多腺苷酸化伴随甲状腺激素消耗。

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In thyroid hormone-depleted rats, the rate of transcription of the growth hormone (GH) gene in the anterior pituitary gland is lower than the rate in euthyroid controls, and there is a corresponding reduction in the abundance of the GH mRNA. Concomitantly, the poly(A) tail of the GH mRNA increases in length. Examination of nuclear RNA from anterior pituitary glands of control and thyroid hormone-depleted rats revealed no difference in the length of pre-mRNAs containing the first and last introns of the GH gene. However, mature nuclear GH RNA is differentially polyadenylated in euthyroid and hypothyroid animals. We suggest that the extent of polyadenylation of the GH transcript is regulated in the cell nucleus concomitant with or subsequent to the splicing of the pre-mRNA. Experiments with anterior pituitary gland explant cultures demonstrated that the GH mRNA from thyroid hormone-depleted rats is more stable than its euthyroid counterpart and that the poly(A) tail may contribute to the differential stability of free GH ribonucleoproteins.
机译：在甲状腺激素缺乏的大鼠中，垂体前叶中生长激素（GH）基因的转录速率低于正常甲状腺对照组的速率，并且GH mRNA的丰度相应降低。同时，GH mRNA的poly（A）尾巴长度增加。从对照组和甲状腺激素耗竭大鼠的垂体前叶核RNA的检查显示，含有GH基因第一个和最后一个内含子的前mRNA的长度没有差异。然而，在正常甲状腺和甲状腺功能减退的动物中，成熟的核GH RNA是差异聚腺苷酸化的。我们建议，GH转录物的多腺苷酸化程度是在剪接pre-mRNA的过程中或之后进行调节的。垂体前叶外植体培养的实验表明，甲状腺激素耗竭大鼠的GH mRNA比其正常甲状腺的GH mRNA更稳定，而poly（A）尾巴可能有助于游离GH核糖核蛋白的差异稳定性。

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