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首页> 外文期刊>Molecular and Cellular Biology >Endogenous Fluctuations of DNA Topology in the Chloroplast of Chlamydomonas reinhardtii
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Endogenous Fluctuations of DNA Topology in the Chloroplast of Chlamydomonas reinhardtii

机译：莱茵衣藻叶绿体中DNA拓扑的内源性波动

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DNA supercoiling in the chloroplast of the unicellular green algaChlamydomonas reinhardtii was found to change with a diurnal rhythm in cells growing in alternating 12-h dark–12-h light periods. Highest and lowest DNA superhelicities occurred at the beginning and towards the end of the 12-h light periods, respectively. The fluctuations in DNA supercoiling occurred concurrently and in the same direction in two separate parts of the chloroplast genome, one containing the genes psaB, rbcL, andatpA and the other containing the atpB gene. Fluctuations were not confined to transcribed DNA regions, indicating simultaneous changes in DNA conformation all over the chloroplast genome. Because the diurnal fluctuations persisted in cells kept in continuous light, DNA supercoiling is judged to be under endogenous control. The endogenous fluctuations in chloroplast DNA topology correlated tightly with the endogenous fluctuations of overall chloroplast gene transcription and with those of the pool sizes of most chloroplast transcripts analyzed. This result suggests that DNA superhelical changes have a role in the regulation of chloroplast gene expression in Chlamydomonas.
机译：发现单细胞绿藻莱茵衣藻的叶绿体中的DNA超螺旋随着昼夜节律的变化而变化，该昼夜节律在黑暗的12小时至12小时的交替光照下生长。最高和最低的DNA超螺旋性分别发生在12小时光照周期的开始和结束时。 DNA超螺旋的波动同时发生在叶绿体基因组的两个不同部分中，并且方向相同，一个部分包含基因 psaB ， rbcL 和 atpA ，另一个包含 atpB 基因。波动不限于转录的DNA区域，这表明在整个叶绿体基因组中DNA构象的同时变化。由于昼夜波动在持续光照下的细胞中持续存在，因此可以认为DNA超螺旋处于内源性控制之下。叶绿体DNA拓扑结构的内源性波动与整体叶绿体基因转录的内源性波动以及所分析的大多数叶绿体转录本的库大小紧密相关。该结果表明DNA超螺旋变化在衣藻中叶绿体基因表达的调节中起作用。

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