Human Topoisomerase I Promotes Initiation of Simian Virus 40 DNA Replication In Vitro

Pamela W. Trowbridge; Rupa Roy; Daniel T. Simmons

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Human Topoisomerase I Promotes Initiation of Simian Virus 40 DNA Replication In Vitro

机译：人类拓扑异构酶I促进猿猴病毒40 DNA体外复制的启动。

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Addition of purified human topoisomerase I (topo I) to simian virus 40 T antigen-driven in vitro DNA replication reactions performed with topo I-deficient extracts results in a greater than 10-fold stimulation of completed molecules as well as a more than 3-fold enhancement of overall DNA replication. To further characterize this stimulation, we first demonstrate that bovine topo I but not Escherichia coli topo I can also enhance DNA replication. By using several human topo I mutants, we show that a catalytically active form of topo I is required. To delineate whether topo I influences the initiation or the elongation step of replication, we performed delayed pulse, pulse-chase, and delayed pulse-chase experiments. The results illustrate that topo I cannot promote the completion of partially replicated molecules but is needed from the beginning of the reaction to initiate replication. Competitive inhibition experiments with the topo I binding T antigen fragment 1-246T and a catalytically inactive topo I mutant suggest that part of topo I’s stimulation of replication is mediated through a direct interaction with T antigen. Collectively, our data indicate that topo I enhances the synthesis of fully replicated DNA molecules by forming essential interactions with T antigen and stimulating initiation.

机译：将纯化的人类拓扑异构酶I（拓扑I）添加到猿猴病毒40 T抗原驱动的体外DNA复制反应中，该拓扑DNA缺陷提取物可产生超过10倍的完整分子刺激，以及超过3倍的刺激。整体DNA复制的倍数增强。为了进一步表征这种刺激，我们首先证明了牛topo I而不是大肠杆菌 topo I也可以增强DNA复制。通过使用几种人类topo I突变体，我们表明topo I的催化活性形式是必需的。为了描述topo I是否影响复制的起始步骤或延伸步骤，我们进行了延迟脉冲，脉冲追踪和延迟脉冲追踪实验。结果表明，topo I不能促进部分复制分子的完成，但是从反应开始就需要它来启动复制。通过与topo I结合的T抗原片段1-246T和催化失活的topo I突变体进行的竞争性抑制实验表明，topo I的复制刺激作用的一部分是通过与T抗原的直接相互作用来介导的。总体而言，我们的数据表明topo I通过与T抗原形成必需的相互作用并刺激启动来增强完全复制的DNA分子的合成。 展开▼

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《Molecular and Cellular Biology》 |1999年第3期|共9页

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Pamela W. Trowbridge; Rupa Roy; Daniel T. Simmons;
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中图分类细胞生物学;

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1. Human Topoisomerase I Promotes Initiation of Simian Virus 40 DNA Replication In Vitro [J] . Pamela W. Trowbridge, Rupa Roy, Daniel T. Simmons Molecular and Cellular Biology . 1999,第3期

机译：人类拓扑异构酶I促进猿猴病毒40 DNA体外复制的启动。

2. Initiation of simian virus 40 DNA replication in vitro: aphidicolin causes accumulation of early-replicating intermediates and allows determination of the initial direction of DNA synthesis. [J] . R S Decker, M Yamaguchi, R Possenti, Molecular and Cellular Biology . 1986,第11期

机译：猿猴病毒40 DNA体外复制的启动：蚜虫菌素引起早期复制中间体的积累，并可以确定DNA合成的初始方向。

3. Effect of ICRF-193, a novel DNA topoisomerase II inhibitor, on simian virus 40 DNA and chromosome replication in vitro. [J] . Y Ishimi, R Ishida, T Andoh Molecular and Cellular Biology . 1992,第9期

机译：新型DNA拓扑异构酶II抑制剂ICRF-193对猿猴病毒40 DNA和染色体复制的影响。

4. The Epstein-Barr Virus Protein Kinase BGLF4 Integrates DNA Damage Response and Mitotic Phosphorylation Signaling to Promote Virus Replication [C] . Renfeng Li, Raja Sekhar Nirujogi, Sneha Pinto, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：Epstein-Barr病毒蛋白激酶BGLF4将DNA损伤反应和有丝分裂磷酸化信号传导整合以促进病毒复制

5. Inhibitory activity of colicins against clonal diarrheagenic Escherichia coli strains and in vitro cytotoxicity of nisin, pediocin, colicins E1, E3, E6, E7, and K, against vero monkey kidney cells and simian virus 40-transfected human colon cells. [D] . Murinda, Shelton Edzayi. 1998

机译：大肠菌素对克隆性腹泻性大肠杆菌菌株的抑制活性以及乳酸链球菌肽，pediocin，大肠菌素E1，E3，E6，E7和K对vero猴肾细胞和猿猴病毒40转染的人结肠细胞的体外细胞毒性。

6. Human Topoisomerase I Promotes Initiation of Simian Virus 40 DNA Replication In Vitro [O] . Pamela W. Trowbridge, Rupa Roy, Daniel T. Simmons 1999

机译：人类拓扑异构酶I促进猿猴病毒40 DNA体外复制的启动。

7. Human Topoisomerase I Promotes Initiation of Simian Virus 40 DNA Replication In Vitro [O] . Trowbridge, Pamela W., Roy, Rupa, Simmons, Daniel T. 1999

机译：人类拓扑异构酶I促进猿猴病毒40 DNA体外复制的启动。

8. In vivo and in vitro replication studies of a mutant SV40 virus isolated from transformed human cells. [R] . Maulbecker, C. C. 1989

机译：从转化的人细胞分离的突变体sV40病毒的体内和体外复制研究。

1. IL-10、IL-4及其受体启动子在人类单纯疱疹病毒1型激活卡波济肉瘤相关疱疹病毒复制过程中的启动活性分析 [J] . 吕志刚 ,卢春 ,秦娣 . 南京医科大学学报（自然科学版） . 2008,第002期

2. 克隆猿猴病毒40增强子修饰人乙酰肝素酶基因核心启动子及活性分析 [J] . 王永 ,陈晓鹏 . 东南大学学报（医学版） . 2012,第001期

3. 1型人类免疫缺陷病毒体外复制、检测系统的建立及评价 [J] . 李杰 ,陈佩玉 ,郑毅 . 现代实用医学 . 2016,第012期

4. 双启动子调控的条件复制腺病毒制备及其体外抑瘤作用 [J] . 任继鸿 ,龙敏 ,温志远 . 现代肿瘤医学 . 2010,第005期

5. 含survivin启动子重组的条件复制腺病毒联合顺铂对卵巢癌株HO-8910体外作用的实验研究 [J] . 张蓓 ,刘嘉茵 ,韩素萍 . 徐州医学院学报 . 2008,第002期

6. 人La蛋白丝氨酸366位磷酸化促进体外乙肝病毒的复制与表达 [C] . TANG Jing ,汤静 ,HEISE Tilman . 第十三次全国临床药理学学术大会 . 2012

7. 猿猴空泡病毒40（SV40）T基因的克隆与表达及其多抗在转基因小鼠中的应用 [A] . 孙海燕 . 2004

1. 猿猴亚家族C腺病毒SAdV-40、-31和-34及其应用 [P] . 中国专利： CN102131920B . 2013.11.06

2. 猿猴亚家族C腺病毒SAdV-40、-31和-34及其应用 [P] . 中国专利： CN102131920A . 2011-07-20

3. Genetically engineered hepatitis c virus (hcv) nucleic acid clone, plasmid clone that houses a life-size hcv cdna, dna or rna transcribed from the full-length hcv cdna, transfected and in vitro infected host cell line with hcv, non-human animal infected with hcv, hcv viral particle, in vitro test system, hcv cell free, hcv vaccine and hcv test set and processes for identifying a cell line that is permissive and an animal that is permissive for hcv infection, to select hcv with adaptive mutations that allow higher levels of hcv replication in a permissive cell line, to infect an animal with hcv, to spread hcv in vitro, to transduce an animal susceptible to hcv infection with a heterologous gene, to produce hcv viral particles, hcv particle proteins and hcv antibodies, to separate agents capable of to modulate hcv replication, to prepare a hcv nucleic acid and to detect hcv antibodies in a biological sample from a patient. [P] . 外国专利： BR9808147A . 2000-03-28

机译：基因工程丙型肝炎病毒（hcv）核酸克隆，质粒克隆，其中包含从全长hcv cdna转录而来的真人大小的hcv cdna，dna或rna，并通过非人类动物的hcv转染和体外感染宿主细胞系被hcv感染，hcv病毒颗粒，体外测试系统，无hcv细胞，hcv疫苗和hcv测试仪的感染以及鉴定允许的细胞系和允许感染hvv的动物的过程，以选择具有以下适应性突变的hcv：允许在允许的细胞系中进行更高水平的hvv复制，以hvv感染动物，在体外传播hvv，用异源基因转导易感染hvv的动物，产生hvv病毒颗粒，hvv颗粒蛋白和hvv抗体分离能够调节hcv复制的试剂，制备hcv核酸并检测患者生物样品中的hcv抗体。

4. PREPARATION OF SIMIAN VIRUS 40DNA DEVOID OF REPLICATION STARTING POINT [P] . 外国专利： JPS61111689A . 1986-05-29

机译：克隆复制起点的猿猴病毒40DNA的制备

5. HUMAN PROSTATIC CELL LINES IMMORTALIZED BY ADENOVIRUS 12-SIMIAN VIRUS 40 (AD12/SV40) HYBRID VIRUS [P] . 外国专利： CA2187099C . 1999-11-09

机译：腺病毒12-猿病毒40（AD12 / SV40）混合病毒固定化的人类前列腺细胞系

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Human Topoisomerase I Promotes Initiation of Simian Virus 40 DNA Replication In Vitro

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