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首页> 外文期刊>Molecular and Cellular Biology >Properties of an Intergenic Terminator and Start Site Switch That Regulate IMD2 Transcription in Yeast
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Properties of an Intergenic Terminator and Start Site Switch That Regulate IMD2 Transcription in Yeast

机译：调控IMD2转录的酵母基因间终止子和起始位点开关的特性

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The IMD2 gene in Saccharomyces cerevisiae is regulated by intracellular guanine nucleotides. Regulation is exerted through the choice of alternative transcription start sites that results in synthesis of either an unstable short transcript terminating upstream of the start codon or a full-length productive IMD2 mRNA. Start site selection is dictated by the intracellular guanine nucleotide levels. Here we have mapped the polyadenylation sites of the upstream, unstable short transcripts that form a heterogeneous family of RNAs of ≈200 nucleotides. The switch from the upstream to downstream start sites required the Rpb9 subunit of RNA polymerase II. The enzyme's ability to locate the downstream initiation site decreased exponentially as the start was moved downstream from the TATA box. This suggests that RNA polymerase II's pincer grip is important as it slides on DNA in search of a start site. Exosome degradation of the upstream transcripts was highly dependent upon the distance between the terminator and promoter. Similarly, termination was dependent upon the Sen1 helicase when close to the promoter. These findings extend the emerging concept that distinct modes of termination by RNA polymerase II exist and that the distance of the terminator from the promoter, as well as its sequence, is important for the pathway chosen.
机译：酿酒酵母中的 IMD2 基因受细胞内鸟嘌呤核苷酸的调控。通过选择其他转录起始位点来进行调节，从而导致合成不稳定的短转录终止于起始密码子的上游或全长生产性 IMD2 mRNA。起始位点的选择由细胞内鸟嘌呤核苷酸水平决定。在这里，我们绘制了上游，不稳定的短转录本的聚腺苷酸化位点，这些转录本形成了一个约200个核苷酸的RNA异质家族。从上游到下游起始位点的转换需要RNA聚合酶II的Rpb9亚基。随着起始点从TATA盒向下游移动，酶定位下游起始位点的能力呈指数下降。这表明RNA聚合酶II的钳夹性很重要，因为它在DNA上滑动以寻找起始位点。上游转录物的外来体降解高度依赖于终止子和启动子之间的距离。同样，当接近启动子时，终止依赖于Sen1解旋酶。这些发现扩展了新兴的概念，即存在RNA聚合酶II终止模式的独特方式，终止子与启动子的距离及其序列对于所选途径很重要。

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