Protein Tyrosine Phosphatase α Phosphotyrosyl-789 Binds BCAR3 To Position Cas for Activation at Integrin-Mediated Focal Adhesions

Guobin Sun; Suzanne Y. S. Cheng; Min Chen; Chinten James Lim; Catherine J. Pallen

首页> 外文期刊>Molecular and Cellular Biology >Protein Tyrosine Phosphatase α Phosphotyrosyl-789 Binds BCAR3 To Position Cas for Activation at Integrin-Mediated Focal Adhesions

【24h】

Protein Tyrosine Phosphatase α Phosphotyrosyl-789 Binds BCAR3 To Position Cas for Activation at Integrin-Mediated Focal Adhesions

机译：蛋白酪氨酸磷酸酶α磷酸酪氨酰-789结合BCAR3定位cas，以在整合素介导的粘着斑处活化

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Integrin-mediated focal adhesions connect the extracellular matrix and cytoskeleton to regulate cell responses, such as migration. Protein tyrosine phosphatase α (PTPα) regulates integrin signaling, focal adhesion formation, and migration, but its roles in these events are incompletely understood. The integrin-proximal action of PTPα activates Src family kinases, and subsequent phosphorylation of PTPα at Tyr789 acts in an unknown manner to promote migration. PTPα-null cells were used in reconstitution assays to distinguish PTPα-Tyr789-dependent signaling events. This showed that PTPα-Tyr789 regulates the localization of PTPα and the scaffolding protein Cas to adhesion sites where Cas interacts with and is phosphorylated by Src to initiate Cas signaling. Linking these events, we identify BCAR3 as a molecular connector of PTPα and Cas, with phospho-Tyr789 PTPα serving as the first defined cellular ligand for the BCAR3 SH2 domain that recruits BCAR3-Cas to adhesions. Our findings reveal a novel role of PTPα in integrin-induced adhesion assembly that enables Src-mediated activation of the pivotal function of Cas in migration.

机译：整联蛋白介导的粘着连接将细胞外基质和细胞骨架连接起来，以调节细胞反应，例如迁移。蛋白酪氨酸磷酸酶α（PTPα）调节整联蛋白信号传导，粘着斑形成和迁移，但其在这些事件中的作用尚不完全清楚。 PTPα的整联蛋白近端作用激活了Src家族激酶，随后Tyr789处PTPα的磷酸化以未知方式起作用，以促进迁移。 PTPα-null细胞用于重组测定，以区分PTPα-Tyr789依赖性信号事件。这表明PTPα-Tyr789调节PTPα和支架蛋白Cas的定位，使Cas与Cas相互作用并被Src磷酸化以启动Cas信号传导。链接这些事件，我们将BCAR3鉴定为PTPα和Cas的分子连接体，磷酸Tyr789PTPα充当BCAR3 SH2域的第一个定义的细胞配体，该结构使BCAR3-Cas募集到粘连。我们的发现揭示了PTPα在整联蛋白诱导的粘附装配中的新作用，该作用使Src介导的Cas的关键功能在迁移中激活。

著录项

来源
《Molecular and Cellular Biology》 |2012年第18期|共14页
作者
Guobin Sun; Suzanne Y. S. Cheng; Min Chen; Chinten James Lim; Catherine J. Pallen;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Interactions of the Protein-tyrosine Phosphatase-α with the Focal Adhesion Targeting Domain of Focal Adhesion Kinase Are Involved in Interleukin-1 Signaling in Fibroblasts [J] . Qin Wang, Yongqiang Wang, Dominik Fritz, The Journal of biological chemistry . 2014,第26期

机译：蛋白质 - 酪氨酸磷酸酶-α与局灶性粘附激酶的焦粘连靶向结构域的相互作用参与成纤维细胞中的白细胞介素-1信号传导
2. Regulation of integrin-mediated T cell adhesion by the transmembrane protein tyrosine phosphatase CD45. [J] . Shenoi H, Seavitt J, Zheleznyak A, The Journal of Immunology: Official Journal of the American Association of Immunologists . 1999,第12期

机译：跨膜蛋白酪氨酸磷酸酶CD45调节整合素介导的T细胞粘附。
3. Ionizing radiation modules of the expression and tyrosine phosphorylation of the focal adhesion-associated proteins focal adhesion kinase (FAK) and its substrates p130cas and paxillin in A549 human lung carcinoma cells in vitro. [J] . Beinke C, Van Beuningen D, Cordes N International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations . 2003,第9期

机译：电离辐射模块在体外对A549人肺癌细胞中黏着斑相关蛋白局灶性粘附激酶（FAK）及其底物p130cas和paxillin的表达和酪氨酸磷酸化的影响。
4. Actinodaphnine and Rutacridone as New T-Cell Protein Tyrosine Phosphatase Inhibitors for Drug Development of Obesity [C] . Y Fitrianingrum, D Indarto, R Kusumawati Annual Basic Science International Conference . 2019

机译：Actinodaphnine和Rutacridone作为新的T细胞蛋白酪氨酸磷酸酶抑制剂，用于肥胖的药物发育
5. The focal adhesion protein paxillin is necessary for the activation of focal adhesion kinase. [D] . Wade, Ramon. 2003

机译：粘着斑蛋白paxillin对于激活粘着斑激酶是必需的。
6. Identification of p130Cas as a substrate of Yersinia YopH (Yop51) a bacterial protein tyrosine phosphatase that translocates into mammalian cells and targets focal adhesions. [O] . D S Black, J B Bliska 1997

机译：鉴定p130Cas作为耶尔森氏菌YopH（Yop51）的底物耶尔森氏菌YypH是一种细菌蛋白酪氨酸磷酸酶可转移到哺乳动物细胞中并靶向粘着斑。
7. Association of the tensin N-terminal protein-tyrosine phosphatase domain with the alpha isoform of protein phosphatase-1 in focal adhesions [O] . Eto Masumi, Kirkbride Jason, Elliott Elizabeth, 2007

机译：张力蛋白N-末端蛋白酪氨酸磷酸酶结构域与粘着斑中蛋白磷酸酶-1的α同种型的关联

Protein Tyrosine Phosphatase α Phosphotyrosyl-789 Binds BCAR3 To Position Cas for Activation at Integrin-Mediated Focal Adhesions

摘要

著录项

相似文献

相关主题

期刊订阅