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Triple Decoding of Hepatitis C Virus RNA by Programmed Translational Frameshifting

机译：通过程序翻译移码法对丙型肝炎病毒RNA的三重解码

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Ribosomes can be programmed to shift from one reading frame to another during translation. Hepatitis C virus (HCV) uses such a mechanism to produce F protein from the ?2/+1 reading frame. We now report that the HCV frameshift signal can mediate the synthesis of the core protein of the zero frame, the F protein of the ?2/+1 frame, and a 1.5-kDa protein of the ?1/+2 frame. This triple decoding function does not require sequences flanking the frameshift signal and is apparently independent of membranes and the synthesis of the HCV polyprotein. Two consensus ?1 frameshift sequences in the HCV type 1 frameshift signal facilitate ribosomal frameshifts into both overlapping reading frames. A sequence which is located immediately downstream of the frameshift signal and has the potential to form a double stem-loop structure can significantly enhance translational frameshifting in the presence of the peptidyl-transferase inhibitor puromycin. Based on these results, a model is proposed to explain the triple decoding activities of the HCV ribosomal frameshift signal.
机译：核糖体可以编程为在翻译过程中从一个阅读框转移到另一个阅读框。丙型肝炎病毒（HCV）使用这种机制从α2/ + 1阅读框中产生F蛋白。我们现在报道，HCV移码信号可以介导零帧核心蛋白，λ2/ + 1帧的F蛋白和λ1/ + 2帧的1.5kDa蛋白的合成。这种三重解码功能不需要移码信号旁的序列，并且显然与膜和HCV多蛋白的合成无关。 HCV 1型移码信号中的两个共有λ1移码序列有助于将核糖体移码到两个重叠的阅读框中。在肽基转移酶抑制剂嘌呤霉素的存在下，紧接移码信号下游并具有形成双茎环结构的潜力的序列可以显着增强翻译移码。基于这些结果，提出了一个模型来解释HCV核糖体移码信号的三重解码活动。

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