• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Eukaryotic Translational Coupling in UAAUG Stop-Start Codons for the Bicistronic RNA Translation of the Non-Long Terminal Repeat Retrotransposon SART1
【24h】

Eukaryotic Translational Coupling in UAAUG Stop-Start Codons for the Bicistronic RNA Translation of the Non-Long Terminal Repeat Retrotransposon SART1

机译：非长末端重复反转录转座子SART1的双顺反子RNA的UAAUG停止密码子中的真核翻译偶联。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Most eukaryotic cellular mRNAs are monocistronic; however, many retroviruses and long terminal repeat (LTR) retrotransposons encode multiple proteins on a single RNA transcript using ribosomal frameshifting. Non-long terminal repeat (non-LTR) retrotransposons are considered the ancestor of LTR retrotransposons and retroviruses, but their translational mechanism of bicistronic RNA remains unknown. We used a baculovirus expression system to produce a large amount of the bicistronic RNA of SART1, a non-LTR retrotransposon of the silkworm, and were able to detect the second open reading frame protein (ORF2) by Western blotting. The ORF2 protein was translated as an independent protein, not as an ORF1-ORF2 fusion protein. We revealed by mutagenesis that the UAAUG overlapping stop-start codon and the downstream RNA secondary structure are necessary for efficient ORF2 translation. Increasing the distance between the ORF1 stop codon and the ORF2 start codon decreased translation efficiency. These results are different from the eukaryotic translation reinitiation mechanism represented by the yeast GCN4 gene, in which the probability of reinitiation increases as the distance between the two ORFs increases. The translational mechanism of SART1 ORF2 is analogous to translational coupling observed in prokaryotes and viruses. Our results indicate that translational coupling is a general mechanism for bicistronic RNA translation.
机译：大多数真核细胞mRNA是单顺反子;然而，许多逆转录病毒和长末端重复（LTR）逆转座子使用核糖体移码在单个RNA转录物上编码多种蛋白质。非长末端重复（非LTR）逆转座子被认为是LTR逆转座子和逆转录病毒的祖先，但其双顺反子RNA的翻译机制仍然未知。我们使用杆状病毒表达系统产生了大量SART1的双顺反子RNA，SART1是家蚕的非LTR反转录转座子，并且能够通过Western印迹检测第二个开放阅读框蛋白（ORF2）。 ORF2蛋白被翻译成独立蛋白，而不是ORF1-ORF2融合蛋白。通过诱变我们揭示了UAAUG重叠的终止-起始密码子和下游RNA二级结构对于有效的ORF2翻译是必需的。 ORF1终止密码子和ORF2起始密码子之间的距离增加会降低翻译效率。这些结果不同于以酵母GCN4基因为代表的真核翻译重新启动机制，其中随着两个ORF之间的距离增加，重新启动的可能性增加。 SART1 ORF2的翻译机制类似于在原核生物和病毒中观察到的翻译偶联。我们的结果表明翻译偶联是双顺反子RNA翻译的一般机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号