...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Liver Receptor Homolog 1 Is a Negative Regulator of the Hepatic Acute-Phase Response
【24h】

Liver Receptor Homolog 1 Is a Negative Regulator of the Hepatic Acute-Phase Response

机译：肝受体同源物1是肝急性期反应的负调节剂。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The orphan nuclear receptor liver receptor homolog 1 (LRH-1) has been reported to play an important role in bile acid biosynthesis and reverse cholesterol transport. Here, we show that LRH-1 is a key player in the control of the hepatic acute-phase response. Ectopic expression of LRH-1 with adenovirus resulted in strong inhibition of both interleukin-6 (IL-6)- and IL-1β-stimulated haptoglobin, serum amyloid A, and fibrinogen β gene expression in hepatocytes. Furthermore, induction of the hepatic inflammatory response was significantly exacerbated in HepG2 cells expressing short hairpin RNA targeting LRH-1 expression. Moreover, transient-transfection experiments and electrophoretic mobility shift and chromatin immunoprecipitation assays revealed that LRH-1 regulates this cytokine-elicited inflammatory response by, at least in part, antagonizing the CCAAT/enhancer binding protein β signaling pathway. Finally, we show, by using LRH-1 heterozygous mice, that LRH-1 is involved in the control of the inflammatory response at the hepatic level in vivo. Taken together, our results outline an unexpected role for LRH-1 in the modulation of the hepatic acute-phase response.
机译：据报道，孤儿核受体肝受体同源物1（LRH-1）在胆汁酸的生物合成和胆固醇逆向转运中起重要作用。在这里，我们显示LRH-1是控制肝急性期反应的关键因素。 LRH-1与腺病毒异位表达可强烈抑制肝细胞中白介素6（IL-6）和IL-1β刺激的触珠蛋白，血清淀粉样蛋白A和纤维蛋白原β基因的表达。此外，在表达靶向LRH-1表达的短发夹RNA的HepG2细胞中，肝炎性反应的诱导显着加剧。此外，瞬时转染实验以及电泳迁移率变化和染色质免疫沉淀试验表明，LRH-1通过至少部分拮抗CCAAT /增强子结合蛋白β信号传导途径来调节这种细胞因子引起的炎症反应。最后，我们显示通过使用LRH-1杂合小鼠，LRH-1参与了体内肝脏水平上炎症反应的控制。综上所述，我们的结果概述了LRH-1在调节肝急性期反应中的出乎意料的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号