• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Tpa1p Is Part of an mRNP Complex That Influences Translation Termination, mRNA Deadenylation, and mRNA Turnover in Saccharomyces cerevisiae
【24h】

Tpa1p Is Part of an mRNP Complex That Influences Translation Termination, mRNA Deadenylation, and mRNA Turnover in Saccharomyces cerevisiae

机译：Tpa1p是mRNP复合体的一部分，该复合体影响啤酒酵母中的翻译终止，mRNA腺苷酸化和mRNA转换。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this report, we show that the Saccharomyces cerevisiae protein Tpa1p (for termination and polyadenylation) influences translation termination efficiency, mRNA poly(A) tail length, and mRNA stability. Tpa1p is encoded by the previously uncharacterized open reading frame YER049W. Yeast strains carrying a deletion of the TPA1 gene (tpa1Δ) exhibited increased readthrough of stop codons, and coimmunoprecipitation assays revealed that Tpa1p interacts with the translation termination factors eRF1 and eRF3. In addition, the tpa1Δ mutation led to a 1.5- to 2-fold increase in the half-lives of mRNAs degraded by the general 5′→3′ pathway or the 3′→5′ nonstop decay pathway. In contrast, this mutation did not have any affect on the nonsense-mediated mRNA decay pathway. Examination of mRNA poly(A) tail length revealed that poly(A) tails are longer than normal in a tpa1Δ strain. Consistent with a potential role in regulating poly(A) tail length, Tpa1p was also found to coimmunoprecipitate with the yeast poly(A) binding protein Pab1p. These results suggest that Tpa1p is a component of a messenger ribonucleoprotein complex bound to the 3′ untranslated region of mRNAs that affects translation termination, deadenylation, and mRNA decay.
机译：在此报告中，我们显示了酿酒酵母（Saccharomyces cerevisiae）蛋白质Tpa1p（用于 t 终止和 p oly a 腺苷酸化）影响翻译终止效率，mRNA poly（A）尾巴长度和mRNA稳定性。 Tpa1p由先前未表征的开放阅读框YER049W编码。带有 TPA1 基因（ tpa1 Δ）缺失的酵母菌株显示终止密码子的通读增加，并且免疫共沉淀分析表明Tpa1p与翻译终止因子eRF1和eRF3相互作用。此外， tpa1 Δ突变导致通过一般5'→3'途径或3'→5'不间断衰变降解的mRNA的半衰期增加1.5到2倍途径。相反，该突变对无义介导的mRNA衰变途径没有任何影响。对mRNA poly（A）尾巴长度的检查显示，在 tpa1 Δ菌株中，poly（A）尾巴比正常长。与调节poly（A）尾巴长度的潜在作用一致，还发现Tpa1p与酵母poly（A）结合蛋白Pab1p共免疫沉淀。这些结果表明，Tpa1p是信使核糖核蛋白复合物的一个成分，该复合物与mRNA的3'非翻译区结合，影响翻译终止，腺苷酸化和mRNA降解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号