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首页> 外文期刊>Molecular and Cellular Biology >BSF Binds Specifically to the bicoidmRNA 3′ Untranslated Region and Contributes to Stabilization ofbicoid mRNA
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BSF Binds Specifically to the bicoidmRNA 3′ Untranslated Region and Contributes to Stabilization ofbicoid mRNA

机译：BSF特异性结合到bicoidmRNA 3'非翻译区，并有助于稳定bicoid mRNA

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The early stages of Drosophila melanogaster development rely extensively on posttranscriptional forms of gene regulation. Deployment of the anterior body patterning morphogen, the Bicoid protein, requires both localization and translational regulation of the maternal bicoid mRNA. Here we provide evidence that thebicoid mRNA is also selectively stabilized during oogenesis. We identify and isolate a protein, BSF, that binds specifically to IV/V RNA, a minimal form of the bicoid mRNA 3′ untranslated region that supports a normal program of mRNA localization during oogenesis. Mutations that disrupt the BSF binding site in IV/V RNA or substantially reduce the level of BSF protein lead to reduction in IV/V RNA levels, indicating a role for BSF in RNA stabilization. The BSF protein is novel and lacks all of the characterized RNA binding motifs. However, BSF does include multiple copies of the PPR motif, whose function is unknown but appears in other proteins with roles in RNA metabolism.
机译：果蝇发展的早期阶段广泛依赖于转录后形式的基因调控。部署前体模式形态发生因子，Bicoid蛋白，需要母体 bicoid mRNA的定位和翻译调控。在这里，我们提供证据，在卵子发生过程中， bicoid mRNA也被选择性稳定。我们鉴定并分离出一种蛋白质，BSF，它与IV / V RNA特异性结合，后者是 bicoid mRNA 3'非翻译区的最小形式，在卵子发生过程中支持正常的mRNA定位程序。破坏IV / V RNA中BSF结合位点或实质上降低BSF蛋白水平的突变导致IV / V RNA水平降低，表明BSF在RNA稳定中发挥了作用。 BSF蛋白是新颖的，缺少所有特征性的RNA结合基序。但是，BSF确实包含PPR基序的多个副本，其功能未知，但出现在其他在RNA代谢中起作用的蛋白质中。

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