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首页> 外文期刊>Molecular and Cellular Biology >p53 Stimulates TFIID-TFIIA-Promoter Complex Assembly, and p53-T Antigen Complex Inhibits TATA Binding Protein-TATA Interaction
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p53 Stimulates TFIID-TFIIA-Promoter Complex Assembly, and p53-T Antigen Complex Inhibits TATA Binding Protein-TATA Interaction

机译：p53刺激TFIID-TFIIA-启动子复合体装配，并且p53-T抗原复合体抑制TATA结合蛋白-TATA相互作用

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Simian virus 40 large T antigen has been shown to inhibit p53-mediated transcription once tethered to p53-responsive promoters through interaction with p53. In this study we report that p53 stimulates transcription by enhancing the recruitment of the basal transcription factors, TFIIA and TFIID, on the promoter (the DA complex) and by inducing a conformational change in the DA complex. Significantly, we have demonstrated that T antigen inhibits p53-mediated transcription by blocking this ability of p53. We investigated the mechanism for this inhibition and found that DA complex formation was resistant to T-antigen repression when the TFIID-DNA complex was formed prior to addition of p53-T antigen complex, indicating that the T antigen, once tethered to the promoter by p53, targets TFIID. Further, we have shown that the p53-T antigen complex prevents the TATA binding protein from binding to the TATA box. Thus, these data suggest a detailed mechanism by which p53 activates transcription and by which T antigen inhibits p53-mediated transcription.
机译：猿猴病毒40大T抗原已显示一旦通过与p53的相互作用而束缚在p53响应性启动子上，便会抑制p53介导的转录。在这项研究中，我们报道p53通过增强启动子（DA复合物）上基础转录因子TFIIA和TFIID的募集并诱导DA复合物中的构象变化来刺激转录。重要的是，我们证明了T抗原通过阻断p53的这种能力来抑制p53介导的转录。我们研究了这种抑制的机制，发现在添加p53-T抗原复合物之前形成TFIID-DNA复合物时，DA复合物的形成对T抗原阻遏具有抵抗力，这表明T抗原一旦通过p53，目标是TFIID。此外，我们已经表明p53-T抗原复合物阻止了TATA结合蛋白与TATA盒结合。因此，这些数据表明了p53激活转录和T抗原抑制p53介导的转录的详细机制。

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