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首页> 外文期刊>Molecular and Cellular Biology >The Tap42-Protein Phosphatase Type 2A Catalytic Subunit Complex Is Required for Cell Cycle-Dependent Distribution of Actin in Yeast
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The Tap42-Protein Phosphatase Type 2A Catalytic Subunit Complex Is Required for Cell Cycle-Dependent Distribution of Actin in Yeast

机译：Tap42蛋白磷酸酶2A型催化亚基复合物是酵母中肌动蛋白细胞周期依赖性分布所必需的

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In Saccharomyces cerevisiae, the Tor proteins mediate a wide spectrum of growth-related cellular processes in response to nutrients. The pleiotropic role of the Tor proteins is mediated, at least in part, by type 2A protein phosphatases (PP2A) and 2A-like protein phosphatases. Tor-mediated signaling activity promotes the interaction of phosphatase-interacting protein Tap42 with PP2A and 2A-like protein phosphatases. The distinct complexes formed between Tap42 and different phosphatases mediate various cellular events and modulate phosphorylation levels of many downstream factors in the Tor pathway in a Tor-dependent and rapamycin-sensitive manner. In this study, we demonstrate that the interaction between Tap42 and the catalytic subunits of PP2A (PP2Ac) is required for cell cycle-dependent distribution of actin. We show that mutations in PP2Ac and Tap42 that perturb the interaction cause random distribution of actin during the cell cycle and that overexpression of the Rho2 GTPase suppresses the actin defects associated with the mutants. Our findings suggest that the Tap42-PP2Ac complex regulates the actin cytoskeleton via a Rho GTPase-dependent mechanism. In addition, we provide evidence that PP2A activity plays a negative role in controlling the actin cytoskeleton and, possibly, in regulation of the G2/M transition of the cell cycle.
机译：在中，Tor蛋白响应营养物质而介导了广泛的与生长相关的细胞过程。 Tor蛋白的多效性作用至少部分地由2A型蛋白磷酸酶（PP2A）和2A样蛋白磷酸酶介导。 Tor介导的信号转导活性促进了磷酸酶相互作用蛋白Tap42与PP2A和2A样蛋白磷酸酶的相互作用。 Tap42和不同磷酸酶之间形成的独特复合物介导各种细胞事件，并以Tor依赖性和雷帕霉素敏感的方式调节Tor途径中许多下游因子的磷酸化水平。在这项研究中，我们证明Tap42和PP2A的催化亚基（PP2Ac）之间的相互作用是肌动蛋白细胞周期依赖性分布所必需的。我们表明扰动相互作用的PP2Ac和Tap42中的突变导致细胞周期中肌动蛋白的随机分布，并且Rho2 GTPase的过表达抑制了与突变体相关的肌动蛋白缺陷。我们的发现表明，Tap42-PP2Ac复合物通过Rho GTPase依赖性机制调节肌动蛋白的细胞骨架。此外，我们提供的证据表明PP2A活性在控制肌动蛋白的细胞骨架，并可能在调节细胞周期的G 2 / M过渡中起着消极作用。

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