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首页> 外文期刊>Molecular and Cellular Biology >Characterization of a Mouse Recombination Hot Spot Locus Encoding a Novel Non-Protein-Coding RNA
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Characterization of a Mouse Recombination Hot Spot Locus Encoding a Novel Non-Protein-Coding RNA

机译：编码新的非蛋白质编码RNA的小鼠重组热点基因座的表征。

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Our current knowledge of recombination hot spot activity in mammalian systems implicates a role for both the primary DNA sequence and the nature of the chromatin domain around it. In mice, the only recombination hot spots mapped to date have been confined to a cluster within the major histocompatibility complex (MHC) region. We present a high resolution analysis of a new recombination hot spot in the mouse genome which maps to mouse chromosome 8 C-D. Haplotype diversity analysis across 40 different strains of mice has enabled us to map recombination breakpoints to a 1-kb interval. This hot spot has a recombination intensity that is 10- to 100-fold above the genome average and has a mean gene conversion tract length of 371 bp. This meiotically active locus happens to be flanked by a transcribed region encoding a non-protein-coding RNA polymerase II transcript and the previously characterized repair site. Many of the primary DNA sequence features that have been reported for the mouse MHC hot spots are also shared by this hot spot locus and in addition, along with three other MHC hot spot loci, we show a new parallel feature of association of the crossover sites with the nuclear matrix.
机译：我们目前对哺乳动物系统中重组热点活性的了解暗示了一级DNA序列及其周围染色质结构域性质的作用。在小鼠中，迄今为止唯一映射的重组热点已被限制在主要组织相容性复合体（MHC）区域内的簇中。我们提出了映射到小鼠染色体8 C-D的小鼠基因组中新的重组热点的高分辨率分析。跨越40种不同品系的小鼠的单倍型多样性分析使我们能够将重组断点定位到1kb的区间。该热点的重组强度是基因组平均值的10到100倍，平均基因转化长度为371 bp。该减数分裂活跃基因座恰好位于编码非蛋白质编码RNA聚合酶II转录本和先前表征的修复位点的转录区的侧翼。此热点基因座还共享了许多小鼠MHC热点报告的主要DNA序列特征，此外，与其他三个MHC热点基因座一起，我们显示了交叉位点关联的新并行特征与核基质。

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