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首页> 外文期刊>Molecular and Cellular Biology >The Schizosaccharomyces pombe HIRA-Like Protein Hip1 Is Required for the Periodic Expression of Histone Genes and Contributes to the Function of Complex Centromeres
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The Schizosaccharomyces pombe HIRA-Like Protein Hip1 Is Required for the Periodic Expression of Histone Genes and Contributes to the Function of Complex Centromeres

机译：裂殖糖酵母HIRA样蛋白Hip1是组蛋白基因的周期性表达所必需的，并且有助于复杂的着丝粒的功能

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HIRA-like (Hir) proteins are evolutionarily conserved and are implicated in the assembly of repressive chromatin. In Saccharomyces cerevisiae, Hir proteins contribute to the function of centromeres. However, S. cerevisiae has point centromeres that are structurally different from the complex centromeres of metazoans. In contrast, Schizosaccharomyces pombe has complex centromeres whose domain structure is conserved with that of human centromeres. Therefore, we examined the functions of the fission yeast Hir proteins Slm9 and the previously uncharacterised protein Hip1. Deletion of hip1+ resulted in phenotypes that were similar to those described previously for slm9Δ cells: a cell cycle delay, synthetic lethality with cdc25-22, and poor recovery from nitrogen starvation. However, while it has previously been shown that Slm9 is not required for the periodic expression of histone H2A, we found that loss of Hip1 led to derepression of core histone genes expression outside of S phase. Importantly, we found that deletion of either hip1+ or slm9+ resulted in increased rates of chromosome loss, increased sensitivity to spindle damage, and reduced transcriptional silencing in the outer centromeric repeats. Thus, S. pombe Hir proteins contribute to pericentromeric heterochromatin, and our data thus suggest that Hir proteins may be required for the function of metazoan centromeres.
机译：HIRA样（Hir）蛋白在进化上是保守的，并与阻抑染色质的组装有关。在酿酒酵母中，Hir蛋白有助于着丝粒的功能。但是， S。酿酒酵母的点着丝点与后生动物的复杂着丝点在结构上有所不同。相反，裂殖酵母（Schizosaccharomyces pombe）具有复杂的着丝粒，其域结构与人类着丝粒的域结构保守。因此，我们检查了裂变酵母Hir蛋白Slm9和以前未表征的蛋白Hip1的功能。删除 hip1 + 产生的表型与之前描述的 slm9 Δ细胞的表型相似：细胞周期延迟，具有合成杀伤力> cdc25-22 ，并且氮饥饿状态恢复较差。然而，尽管先前已经证明组蛋白H2A的周期性表达不需要Slm9，但我们发现Hip1的缺失导致S期之外核心组蛋白基因表达的抑制。重要的是，我们发现删除 hip1 + 或 slm9 + 会导致染色体丢失率增加，对纺锤体损伤敏感，并减少外部着丝粒重复序列中的转录沉默。因此， S。 pombe Hir蛋白有助于着丝粒着丝粒异染色质，因此我们的数据表明，Hir蛋白可能是后生着丝粒功能的必需物质。

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