• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Mechanism of B-Cell Receptor-Induced Phosphorylation and Activation of Phospholipase C-γ2
【24h】

Mechanism of B-Cell Receptor-Induced Phosphorylation and Activation of Phospholipase C-γ2

机译：B细胞受体诱导的磷酸化和磷脂酶C-γ2活化的机制

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phospholipase C-γ2 (PLC-γ2) plays an important role in B-cell signaling. Phosphorylation of various tyrosine residues of PLC-γ2 has been implicated in regulation of its lipase activity. With the use of antibodies specific for each of the putative phosphorylation sites, we have now shown that PLC-γ2 is phosphorylated on Y753, Y759, and Y1217 in response to engagement of the B-cell receptor in Ramos cells, as well as in murine splenic B cells. In cells stimulated maximally via this receptor, the extent of phosphorylation of Y1217 was three times that of Y753 or of Y759. Stimulation of Jurkat T cells or platelets via their immunoreceptors also elicited phosphorylation of Y753 and Y759 but not that of Y1217. A basal level of phosphorylation of Y753 was apparent in unstimulated lymphocytes. The extent of phosphorylation of Y753 and Y759, but not that of Y1217, correlated with the lipase activity of PLC-γ2. Examination of the effects of various pharmacological inhibitors and of RNA interference in Ramos cells suggested that Btk is largely, but not completely, responsible for phosphorylation of Y753 and Y759, whereas phosphorylation of Y1217 is independent of Btk. Finally, phosphorylation of Y1217 and that of Y753 and Y759 occurred on different PLC-γ2 molecules.
机译：磷脂酶C-γ2（PLC-γ2）在B细胞信号传导中起重要作用。 PLC-γ2的各种酪氨酸残基的磷酸化涉及其脂酶活性的调节。通过使用对每个推定的磷酸化位点具有特异性的抗体，我们现在已经显示，响应于Ramos细胞以及鼠中B细胞受体的结合，PLC-γ2在Y753，Y759和Y1217上被磷酸化脾B细胞。在通过该受体最大程度刺激的细胞中，Y1217的磷酸化程度是Y753或Y759的三倍。通过其免疫受体刺激Jurkat T细胞或血小板也引起Y753和Y759的磷酸化，但不引起Y1217的磷酸化。在未刺激的淋巴细胞中，Y753的基本磷酸化水平明显。 Y753和Y759的磷酸化程度，而不是Y1217的磷酸化程度，与PLC-γ2的脂肪酶活性相关。对Ramos细胞中各种药理抑制剂的影响和RNA干扰的研究表明，Btk在很大程度上而非完全地负责Y753和Y759的磷酸化，而Y1217的磷酸化则独立于Btk。最后，Y1217以及Y753和Y759的磷酸化发生在不同的PLC-γ2分子上。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号