• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >The Tup1 Corepressor Directs Htz1 Deposition at a Specific Promoter Nucleosome Marking the GAL1 Gene for Rapid Activation
【24h】

The Tup1 Corepressor Directs Htz1 Deposition at a Specific Promoter Nucleosome Marking the GAL1 Gene for Rapid Activation

机译：Tup1 Corepressor指导Htz1沉积在特定启动子核小体上标记GAL1基因的快速激活。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The SWR1 complex (SWR1-C)-dependent deposition of the histone variant Htz1 on promoter nucleosomes is typical of Saccharomyces cerevisiae genes whose expression is frequently reprogrammed. Although this epigenetic marking is of significant physiological importance, the determinants of Htz1 deposition, the conditions that set off SWR1-C occupancy, and the implications of Htz1 in transcriptional initiation are issues that remain unresolved. In this report, we addressed these questions by investigating the GAL1 promoter. We show that Htz1 is required for efficient Mediator recruitment and transcription only when the GAL1 promoter is under the influence of the Tup1 corepressor. In fact, we show that it is Tup1 that specifies Htz1 deposition for the promoter nucleosome covering the transcription start site. This deposition occurs rapidly following transcriptional repression, and it correlates with a Tup1-independent transient recruitment of the SWR1 complex. We propose that Tup1 cooperates with SWR1-C and specifies Htz1 deposition at GAL1, thereby marking the promoter for rapid neutralization from its repressive effects.
机译：组蛋白变体Htz1在启动子核小体上的SWR1复合物（SWR1-C）依赖性沉积是啤酒酵母基因的典型表达，这些基因的表达经常被重新编程。尽管这种表观遗传学标记具有重要的生理重要性，但Htz1沉积的决定因素，引起SWR1-C占用的条件以及Htz1在转录起始中的影响仍未解决。在此报告中，我们通过研究 GAL1 启动子解决了这些问题。我们显示只有当 GAL1 启动子在Tup1 corepressor的影响下，Htz1才是有效的介体募集和转录所必需的。实际上，我们表明正是Tup1为覆盖转录起始位点的启动子核小体指定了Htz1沉积。这种沉积在转录抑制后迅速发生，并且与SWR1复合体的独立于Tup1的瞬时募集相关。我们建议Tup1与SWR1-C合作，并指定在 GAL1 处的Htz1沉积，从而从其抑制作用中标记启动子以进行快速中和。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号