...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Cdc7-Dbf4 Is a Gene-Specific Regulator of Meiotic Transcription in Yeast
【24h】

Cdc7-Dbf4 Is a Gene-Specific Regulator of Meiotic Transcription in Yeast

机译：Cdc7-Dbf4是酵母减数分裂转录的基因特定调节剂。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Meiosis divides the chromosome number of the cell in half by having two rounds of chromosome segregation follow a single round of chromosome duplication. The first meiotic division is unique in that homologous pairs of sister chromatids segregate to opposite poles. Recent work in budding and fission yeast has shown that the cell cycle kinase, Cdc7-Dbf4, is required for many meiosis-specific chromosomal functions necessary for proper disjunction at meiosis I. This work reveals another role for Cdc7 in meiosis as a gene-specific regulator of the global transcription factor, Ndt80, which is required for exit from pachytene and entry into the meiotic divisions in budding yeast. Cdc7-Dbf4 promotes NDT80 transcription by relieving repression mediated by a complex of Sum1, Rfm1, and a histone deacetylase, Hst1. Sum1 exhibits meiosis-specific Cdc7-dependent phosphorylation, and mass spectrometry analysis reveals a dynamic and complex pattern of phosphorylation events, including four constitutive cyclin-dependent kinase (Cdk1) sites and 11 meiosis-specific Cdc7-Dbf4-dependent sites. Analysis of various phosphorylation site mutants suggests that Cdc7 functions with both Cdk1 and the meiosis-specific kinase Ime2 to control this critical transition point during meiosis.
机译：减数分裂通过在单轮染色体复制后进行两轮染色体分离，将细胞的染色体数减半。第一次减数分裂分裂的独特之处在于同源的姐妹染色单体对分离到相反的两极。萌芽和裂变酵母的最新研究表明，细胞周期激酶Cdc7-Dbf4是减数分裂I适当分离所必需的许多减数分裂特异性染色体功能所必需的。这项工作揭示了Cdc7在减数分裂中的另一种作用是基因特异性。全局转录因子Ndt80的调节子，它是从粗线虫中退出并进入发芽酵母中减数分裂所必需的。 Cdc7-Dbf4通过减轻Sum1，Rfm1和组蛋白脱乙酰基酶Hst1的复合体介导的阻遏作用来促进 NDT80 转录。 Sum1表现出减数分裂特异的Cdc7依赖性磷酸化，质谱分析揭示了磷酸化事件的动态和复杂模式，包括四个组成型细胞周期蛋白依赖性激酶（Cdk1）位点和11个减数分裂特异的Cdc7-Dbf4依赖性位点。对各种磷酸化位点突变体的分析表明，Cdc7与Cdk1和减数分裂特异性激酶Ime2共同控制减数分裂过程中的这一关键转变点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号