...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Distinct Properties of Human HMGN5 Reveal a Rapidly Evolving but Functionally Conserved Nucleosome Binding Protein
【24h】

Distinct Properties of Human HMGN5 Reveal a Rapidly Evolving but Functionally Conserved Nucleosome Binding Protein

机译：人类HMGN5的不同特性揭示了快速进化但功能保守的核小体结合蛋白。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The HMGN family is a family of nucleosome-binding architectural proteins that affect the structure and function of chromatin in vertebrates. We report that the HMGN5 variant, encoded by a gene located on chromosome X, is a rapidly evolving protein with an acidic C-terminal domain that differs among vertebrate species. We found that the intranuclear organization and nucleosome interactions of human HMGN5 are distinct from those of mouse HMGN5 and that the C-terminal region of the protein is the main determinant of the chromatin interaction properties. Despite their apparent differences, both mouse and human HMGN5 proteins interact with histone H1, reduce its chromatin residence time, and can induce large-scale chromatin decompaction in living cells. Analysis of HMGN5 mutants suggests that distinct domains in HMGN5 affect specific steps in the interaction of H1 with chromatin. Elevated levels of either human or mouse HMGN5 affect the transcription of numerous genes, most in a variant-specific manner. Our study identifies HMGN5 as a rapidly evolving vertebrate nuclear protein with species-specific properties. HMGN5 has a highly disordered structure, binds dynamically to nucleosome core particles, modulates the binding of H1 to chromatin, reduces the compaction of the chromatin fiber, and affects transcription.
机译：HMGN家族是一个与核小体结合的建筑蛋白家族，可影响脊椎动物染色质的结构和功能。我们报告说，由位于X染色体上的一个基因编码的HMGN5变异体是一种快速进化的蛋白质，其酸性C末端结构域在脊椎动物之间有所不同。我们发现人HMGN5的核内组织和核小体相互作用与小鼠HMGN5的不同，并且蛋白质的C末端区域是染色质相互作用特性的主要决定因素。尽管它们的表观差异，小鼠和人类HMGN5蛋白都与组蛋白H1相互作用，减少了其染色质停留时间，并可以在活细胞中诱导大规模的染色质分解。 HMGN5突变体的分析表明，HMGN5中的不同结构域会影响H1与染色质相互作用的特定步骤。人或小鼠HMGN5的水平升高都会影响许多基因的转录，大多数以变体特异性方式发生。我们的研究确定HMGN5是具有物种特异性的快速进化的脊椎动物核蛋白。 HMGN5具有高度无序的结构，可动态结合至核小体核心颗粒，调节H1与染色质的结合，降低染色质纤维的紧密度，并影响转录。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号