DGCR14 Induces Il17a Gene Expression through the RORγ/BAZ1B/RSKS2 Complex

Ichiro Takada

首页> 外文期刊>Molecular and Cellular Biology >DGCR14 Induces Il17a Gene Expression through the RORγ/BAZ1B/RSKS2 Complex

【24h】

DGCR14 Induces Il17a Gene Expression through the RORγ/BAZ1B/RSKS2 Complex

机译：DGCR14通过RORγ/ BAZ1B / RSKS2复合体诱导Il17a基因表达

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The Dgcr14/Es2 gene is located in a chromosomal region the loss of which has been associated with DiGeorge syndrome, a cause of immunodeficiency, heart defects, and skeletal abnormalities. However, the role of DGCR14 protein remains to be elucidated. Here, I found that DGCR14 protein acts as a coactivator of RORγt in T_H17 cells. Biochemical purification of the RORγ coregulator complex allowed me to identify the associated DGCR14 protein by matrix-assisted laser desorption ionization–time of flight mass spectrometry. Overexpression of Dgcr14 mRNA enhanced RORγt-mediated transcriptional activity and facilitated T_H17 cell differentiation. Furthermore, knockdown of Dgcr14 reduced Il17a mRNA expression. I also found that DGCR14 associated with ribosomal S6 kinase 2 (RSK2, also called RpS6ka3) and BAZ1B, both of which were recruited to the Il17a promoter during T_H17 cell differentiation. Knockdown of Baz1b or RpS6ka3 also reduced Il17a mRNA expression, and Baz1b knockdown increased transcriptional suppressive histone marks (histone H3K9me3) on the Il17a promoter. My findings showed the roles of DGCR14, RSK2, and BAZ1B in the transcriptional regulation of Il17a mRNA during T_H17 cell differentiation.

机译： Dgcr14 / Es2 基因位于染色体区域，该区域的丢失与DiGeorge综合征有关，后者是免疫缺陷，心脏缺陷和骨骼异常的原因。但是，DGCR14蛋白的作用尚待阐明。在这里，我发现DGCR14蛋白在T _{H 17细胞中作为RORγt的共激活因子。 RORγ调节剂复合物的生化纯化使我能够通过基质辅助激光解吸电离-飞行时间质谱法鉴定相关的DGCR14蛋白。 Dgcr14 mRNA的过表达增强RORγt介导的转录活性并促进T _{H 17细胞分化。此外，敲除Dgcr14减少 Il17a mRNA表达。我还发现DGCR14与核糖体S6激酶2（RSK2，也称为RpS6ka3）和BAZ1B相关，在T _{H 17细胞分化过程中，两者均被募集到 Il17a 启动子。敲除 Baz1b 或 RpS6ka3 也会降低 Il17a mRNA表达，而 Baz1b 敲除会增加转录抑制组蛋白标记（组蛋白H3K9me3 ）在 Il17a 启动子上。我的发现显示了在T _{H 17细胞分化过程中DGCR14，RSK2和BAZ1B在 Il17a mRNA转录调控中的作用。}}}} 展开▼

著录项

来源
《Molecular and Cellular Biology》 |2015年第2期|共12页

作者
Ichiro Takada;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类细胞生物学;

关键词

相似文献

外文文献

中文文献

专利

1. Polypropylenimine dendrimer-induced gene expression changes: the effect of complexation with DNA, dendrimer generation and cell type. [J] . Omidi Y, Hollins AJ, Drayton RM, Journal of drug targeting . 2005,第7期

机译：聚丙烯亚胺树状聚合物诱导的基因表达变化：与DNA的复合作用，树状聚合物的生成和细胞类型的影响。

2. mRNA expression profile of the TNF-alpha system in LH-induced bovine preovulatory follicles and effects of TNF-alpha on gene expression, ultrastructure and expansion of cumulus-oocyte complexes cultured in vitro [J] . Theriogenology . 2017,第期

机译：LH诱导的牛牛瘟卵泡的TNF-α系统的mRNA表达谱和TNF-α对体外培养的基因表达，超微结构和膨胀性的影响

3. Functional expression of ribozymes in Apicomplexa: towards exogenous control of gene expression by inducible RNA-cleavage. [J] . Agop-Nersesian C, Pfahler J, Lanzer M, International Journal for Parasitology . 2008,第6期

机译：螺复合体中核酶的功能性表达：通过诱导性RNA切割实现基因表达的外源控制。

4. Generating Two-Dimensional Repertoire of siRNA Linc-ROR and siRNA mRNA ARF6 from the lincRNA-RoR/miR-145/ARF6 expression Pathway that involved in the progression of Triple Negative Breast Cancer [C] . Arli Aditya Parikesit, Rizki Nurdiansyah International Conference on Chemistry and Material Science . 2018

机译：从Lincrna-ROR / miR-145 / ARF6表达途径产生三维曲线和siRNA mRNA ARF6的二维曲目，参与三重阴性乳腺癌进展

5. A complex program of dopamine-induced gene expression identified using differential-display PCR: Discovery of a novel inducible cyclin. [D] . Berke, Joshua Damien. 1998

机译：使用差异显示PCR鉴定的多巴胺诱导的基因表达的复杂程序：发现新型诱导型细胞周期蛋白。

6. DGCR14 Induces Il17a Gene Expression through the RORγ/BAZ1B/RSKS2 Complex [O] . Ichiro Takada 2015

机译：DGCR14通过RORγ/ BAZ1B / RSKS2复合体诱导Il17a基因表达

7. The orphan nuclear receptor, ROR alpha, regulates gene expression that controls lipid metabolism - Staggerer (sg/sg) mice are resistant to diet-induced obesity [O] . Lau, Patrick, Fitzsimmons, Rebecca L., Raichur, Suryaprakash, 2008

机译：孤儿核受体RORα调节控制脂质代谢的基因表达-Staggerer（sg / sg）小鼠对饮食诱发的肥胖具有抵抗力

1. miR-20a-5p调控结核分枝杆菌诱导巨噬细胞凋亡相关基因表达的研究 [J] . 丁光贵 ,贺星 ,梁娟 . 中国防痨杂志 . 2019,第007期

2. 17α,20β双羟孕酮诱导鲤卵母细胞最终成熟相关基因表达内参基因的筛选 [J] . 史艳艳 ,卢洁 ,王艺磊 . 中国水产科学 . 2014,第005期

3. 骨化三醇诱导锌指蛋白A20抑制下游基因表达延长肝移植大鼠生存期 [J] . 章爱斌 ,郑树森 . 中华普通外科杂志 . 2006,第006期

4. 母体埋植褪黑激素对子一代獭兔内脏组织中RORα基因表达的影响 [J] . 康景 ,封洋 . 中国畜牧杂志 . 2020,第001期

5. 干扰ROR1基因表达可抑制骨肉瘤MG-63细胞的上皮-间质转化 [J] . 王兴文 ,移志刚 ,赵鑫 . 肿瘤 . 2018,第003期

6. 乙烯诱导优异抗晚疫病马铃薯基因型SD20早期响应基因表达 [C] . 杨晓慧 ,陈立 ,杨煜 . 2020中国马铃薯大会 . -1

7. 小鼠脾淋巴细胞中Th17细胞分化诱导及转录因子RORγt、RORα、STAT3、CtBP、Gfil表达 [A] . 柯国琳 . 2014

1. 一种逆境诱导型启动子、逆境诱导型启动子植物表达载体及诱导目标基因表达方法 [P] . 中国专利： CN107881172B . 2021.06.08

2. 一种逆境诱导型启动子、逆境诱导型启动子植物表达载体及诱导目标基因表达方法 [P] . 中国专利： CN107881172A . 2018-04-06

3. Chimeric regulatory region to express genes in plants, expression of a gene cassette tape for inducible expression of a gene to express a weird process gene in a plant plasmidAnd the process for inducible expression of a gene in a plant process for strange inducible expression of a gene in a plant plasmid and strange plant transgenic [P] . 外国专利： BR9408093A . 1997-08-12

机译：用于在植物中表达基因的嵌合调控区，用于在植物质粒中诱导基因表达以表达怪异过程基因的基因盒带表达以及在植物过程中用于基因异常诱导性表达的基因诱导表达的过程在植物质粒和奇怪的植物转基因中

4. CHIMERIC REGULATORY SITE FOR EXPRESSION OF GENES IN PLANTS (VARIANTS), CLASTER FOR GENE EXPRESSION (VARIANTS), CLASTER FOR INDUCIBLE EXPRESSION OF FOREIGN GENE (VARIANTS), METHOD OF GENE EXPRESSION IN PLANT (VARIANTS), METHOD OF INDUCIBLE EXPRESSION OF FOREIGN GENE IN PLANTS (VARIANTS) AND PLASMID (VARIANTS) [P] . 外国专利： RU2142998C1 . 1999-12-20

机译：植物基因表达的嵌合调控位点，基因表达群（变异体），外源基因可诱导表达群，植物中基因表达（变异体）的方法，外源基因无法表达的方法植物（变种）和质粒（变种）

5. A Chemically inducible Promoter Genetic Sequence of a DNA Molecule and a multimer comprising such a construction sequence, GeneticA plant that contains the genetic Construction, a combination of Promoter / Inducer and an expression System for the Plant, which includes the sequence, a transgenic Plant.The use of the sequence that induces the expression of a Gene when it is bound to the sequence and process of expression in Plant [P] . 外国专利： AR003642A1 . 1998-08-05

机译：DNA分子的化学诱导型启动子遗传序列和包含该构建序列的多聚体，包含遗传构建的GeneticA植物，启动子/诱导子和该植物的表达系统的组合，其中包括该序列，一种转基因植物当基因与植物中的序列和表达过程结合时，诱导基因表达的序列的使用

相关主题

DGCR14 Induces Il17a Gene Expression through the RORγ/BAZ1B/RSKS2 Complex

摘要

著录项

相似文献

相关主题

期刊订阅