• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Functional Association between Eyegone and HP1a Mediates wingless Transcriptional Repression during Development
【24h】

Functional Association between Eyegone and HP1a Mediates wingless Transcriptional Repression during Development

机译：Eyegone和HP1a之间的功能关联在发育过程中介导无翅转录抑制。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The eyegone (eyg) gene encodes Eyg, a transcription factor of the Pax family with multiple roles during Drosophila development. Although Eyg has been shown to act as a repressor, nothing is known about the mechanism by which it represses its target genes. Here, we show that Eyg forms a protein complex with heterochromatin protein 1a (HP1a). Both proteins bind to the same chromatin regions on polytene chromosomes and act cooperatively to suppress variegation and mediate gene silencing. In addition, Eyg binds to a wingless (wg) enhancer region, recruiting HP1a to assemble a closed, heterochromatin-like conformation that represses transcription of the wg gene. We describe here the evidence that suggests that Eyg, encoded by eyegone (eyg), represses wingless (wg) during eye development by association with HP1a. We show that Eyg forms a protein complex with HP1a and both proteins colocalize on salivary gland polytene chromosomes. Using position effect variegation (PEV) experiments, we demonstrated that eyg has a dose-dependent effect on heterochromatin gene silencing and identified a genetic interaction with HP1a in this process. We further demonstrated that HP1a binds to the same wg enhancer element as Eyg. DNase I sensitivity assays indicated that this enhancer region has a closed heterochromatin-like conformation, which becomes open in eyg mutants. In these mutants, much less HP1a binds to the wg enhancer region, as shown by ChIP experiments. Furthermore, as previously described for Eyg, a reduction in the amount of HP1a in the eye imaginal disc derepresses wg. Together, our results suggest a model in which Eyg specifically binds to the wg enhancer region, recruiting HP1a to that site. The recruitment of HP1a prevents transcription by favoring a closed, heterochromatin-like structure. Thus, for the first time, we show that HP1a plays a direct role in the repression of a developmentally regulated gene, wg, during Drosophila eye development.
机译： eyegone （ eyg ）基因编码Eyg，这是Pax家族在果蝇发育过程中具有多种作用的转录因子。尽管已证明Eyg可以起阻遏物的作用，但对其阻遏其靶基因的机制尚无知。在这里，我们显示Eyg与异染色质蛋白1a（HP1a）形成蛋白质复合物。两种蛋白都与多态染色体上相同的染色质区域结合，并协同作用以抑制杂色并介导基因沉默。此外，Eyg结合到 wingless （ wg ）增强子区域，募集HP1a组装一个封闭的异染色质样构象，从而抑制 wg 的转录基因。我们在这里描述的证据表明，由 eyegone （ eyg ）编码的Eyg抑制了 wingless （ wg ）与HP1a相关的眼睛发育过程中。我们显示，Eyg与HP1a形成蛋白质复合物，并且这两种蛋白质在唾液腺多烯染色体上共定位。使用位置效应杂色化（PEV）实验，我们证明 eyg 对异染色质基因沉默具有剂量依赖性，并在此过程中确定了与HP1a的遗传相互作用。我们进一步证明，HP1a与Eyg结合相同的 wg 增强子元件。 DNase I敏感性测定表明，该增强子区域具有封闭的异染色质样构象，在 eyg 突变体中开放。如ChIP实验所示，在这些突变体中，很少有HP1a与 wg 增强子区域结合。此外，如先前针对Eyg所述，眼部视盘中HP1a数量的减少会降低 wg 。在一起，我们的结果提出了一种模型，其中Eyg特异性结合 wg 增强子区域，将HP1a募集到该位点。 HP1a的募集通过促进封闭的异染色质样结构来防止转录。因此，我们首次表明，果蝇眼发育过程中HP1a在发育调控基因 wg 的抑制中起直接作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号