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首页> 外文期刊>Molecules >Preparative Isolation of Seven Diterpenoid Alkaloids from Aconitum coreanum by pH-Zone-Refining Counter-Current Chromatography
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Preparative Isolation of Seven Diterpenoid Alkaloids from Aconitum coreanum by pH-Zone-Refining Counter-Current Chromatography

机译：pH-区-分离-逆流色谱法从乌头中制备7种二萜类生物碱

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摘要

The aim of this paper was to seek an efficient method to preparative separation of alkaloid compounds from Aconitum coreanum (Guanbaifu), a well-known traditional Chinese medicinal plant for heart disease. Seven alkaloid compounds were successfully purified by pH-zone-refining counter-current chromatography with two-phase solvent system of petroleum ether–ethyl acetate–methanol–water (5:5:1:9, v/v/v/v), 10 mM triethylamine in upper phase and 10 mM hydrochloric acid in lower phase. From 3.5 g of crude extract, 356 mg of Guanfu base I, 578 mg of Guanfu base A, 74 mg of atisine, 94 mg of Guanfu base F, 423 mg of Guanfu base G, 67 mg of Guanfu base R and 154 mg of Guanfu base P were obtained with the purity of 96.40%, 97.2%, 97.5%, 98.1%, 98.9%, 98.3% and 98.4%. Their chemical structures were identified by TOF-MS and 1H-NMR. This study indicated that pH-zone-refining counter-current chromatography was an efficient method for separating the kind of alkaloids with low absorbance values.
机译：本文的目的是寻找一种有效的方法，从著名的中药心脏病治疗用乌头（冠乌头）中制备生物碱化合物。用石油醚-乙酸乙酯-甲醇-水（5：5：1：9，v / v / v / v）两相溶剂系统通过pH区域精制逆流色谱法成功纯化了7种生物碱化合物，上层为10 mM三乙胺，下层为10 mM盐酸。从3.5克粗提物中，356毫克冠府碱I，578毫克冠府碱A，74毫克牛磺酸，94毫克冠府碱F，423毫克冠府碱G，67毫克冠府碱R和154毫克得到的冠府碱P的纯度为96.40％，97.2％，97.5％，98.1％，98.9％，98.3％和98.4％。通过TOF-MS和 1 H-NMR鉴定其化学结构。这项研究表明，pH区精制逆流色谱法是一种分离低吸光度生物碱的有效方法。

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