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首页> 外文期刊>Molecules >Ginsenoside Re Promotes Osteoblast Differentiation in Mouse Osteoblast Precursor MC3T3-E1 Cells and a Zebrafish Model
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Ginsenoside Re Promotes Osteoblast Differentiation in Mouse Osteoblast Precursor MC3T3-E1 Cells and a Zebrafish Model

机译：人参皂甙Re促进小鼠成骨细胞前体MC3T3-E1细胞和斑马鱼模型中成骨细胞的分化

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Bone homeostasis is tightly regulated to balance bone formation and bone resorption. Many anabolic drugs are used as bone-targeted therapeutic agents for the promotion of osteoblast-mediated bone formation or inhibition of osteoclast-mediated bone resorption. Previous studies showed that ginsenoside Re has the effect of the suppression of osteoclast differentiation in mouse bone-marrow derived macrophages and zebrafish. Herein, we investigated whether ginsenoside Re affects osteoblast differentiation and mineralization in in vitro and in vivo models. Mouse osteoblast precursor MC3T3-E1 cells were used to investigate cell viability, alkaline phosphatase (ALP) activity, and mineralization. In addition, we examined osteoblastic signaling pathways. Ginsenoside Re affected ALP activity without cytotoxicity, and we also observed the stimulation of osteoblast differentiation through the activation of osteoblast markers including runt-related transcription factor 2, type 1 collagen, ALP, and osteocalcin in MC3T3-E1 cells. Moreover, Alizarin red S staining indicated that ginsenoside Re increased osteoblast mineralization in MC3T3-E1 cells and zebrafish scales compared to controls. These results suggest that ginsenoside Re promotes osteoblast differentiation as well as inhibits osteoclast differentiation, and it could be a potential therapeutic agent for bone diseases. View Full-Text
机译：严格调节骨稳态以平衡骨形成和骨吸收。许多合成代谢药物被用作骨靶向治疗剂，以促进成骨细胞介导的骨形成或抑制破骨细胞介导的骨吸收。先前的研究表明，人参皂甙Re具有抑制小鼠骨髓来源的巨噬细胞和斑马鱼中破骨细胞分化的作用。在这里，我们调查了人参皂甙Re是否会在体外和体内模型中影响成骨细胞的分化和矿化。小鼠成骨细胞前体MC3T3-E1细胞用于研究细胞活力，碱性磷酸酶（ALP）活性和矿化作用。此外，我们检查了成骨细胞信号通路。人参皂甙Re影响ALP活性而无细胞毒性，我们还观察到MC3T3-E1细胞中通过激活成骨细胞标志物（包括与矮子相关的转录因子2、1型胶原蛋白，ALP和骨钙蛋白）而刺激成骨细胞分化。此外，茜素红S染色表明人参皂苷Re与对照组相比增加了MC3T3-E1细胞和斑马鱼鳞片中成骨细胞的矿化作用。这些结果表明人参皂甙Re促进成骨细胞分化并抑制破骨细胞分化，它可能是治疗骨病的潜在药物。查看全文

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