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首页> 外文期刊>Molecules >Targeted and Untargeted Metabolomics to Explore the Bioavailability of the Secoiridoids from a Seed/Fruit Extract (Fraxinus angustifolia Vahl) in Human Healthy Volunteers: A Preliminary Study
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Targeted and Untargeted Metabolomics to Explore the Bioavailability of the Secoiridoids from a Seed/Fruit Extract (Fraxinus angustifolia Vahl) in Human Healthy Volunteers: A Preliminary Study

机译：有针对性和无针对性的代谢组学，以探索人类健康志愿者中的种子/水果提取物（Fraxinus angustifolia Vahl）的类胡萝卜素的生物利用度：一项初步研究。

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The bark, seeds, fruits and leaves of the genus Fraxinus (Oleaceae) which contain a wide range of phytochemicals, mostly secoiridoid glucosides, have been widely used in folk medicine against a number of ailments, yet little is known about the metabolism and uptake of the major Fraxinus components. The aim of this work was to advance in the knowledge on the bioavailability of the secoiridoids present in a Fraxinus angustifolia Vahl seed/fruit extract using both targeted and untargeted metabolomic analyses. Plasma and urine samples from nine healthy volunteers were taken at specific time intervals following the intake of the extract and analyzed by UPLC-ESI-QTOF. Predicted metabolites such as tyrosol and ligstroside-aglycone glucuronides and sulfates were detected at low intensity. These compounds reached peak plasma levels 2 h after the intake and exhibited high variability among the participants. The ligstroside-aglycone conjugates may be considered as potential biomarkers of the Fraxinus secoiridoids intake. Using the untargeted approach we additionally detected phenolic conjugates identified as ferulic acid and caffeic acid sulfates, as well as hydroxybenzyl and hydroxyphenylacetaldehyde sulfate derivatives which support further metabolism of the secoiridoids by phase I and (or) microbial enzymes. Overall, the results of this study suggest low uptake of intact secoiridoids from a Fraxinus angustifolia Vahl extract in healthy human volunteers and metabolic conversion by esterases, glycosidases, and phase II sulfo- and glucuronosyl transferases to form smaller conjugated derivatives.
机译：Fraxinus（Oleaceae）的树皮，种子，果实和叶子包含多种植物化学成分，主要是类secrididoid糖苷，已广泛用于民间医学中以对抗多种疾病，但对这种疾病的代谢和摄取知之甚少主要的水曲柳成分。这项工作的目的是利用有针对性的和无目标的代谢组学分析，提高对水曲柳种子/果实提取物中存在的类蛇形素生物利用度的认识。在摄入提取物后的特定时间间隔内，从九位健康志愿者的血浆和尿液样本中进行提取，并通过UPLC-ESI-QTOF进行分析。在低强度下检测到了预测的代谢产物，例如酪醇和麦芽糖苷-苷元葡糖醛酸苷和硫酸盐。这些化合物在摄入后2 h达到峰值血浆水平，并且参与者之间表现出很高的变异性。糖苷-糖苷配位体可以被认为是水曲柳类蛇毒苷摄入量的潜在生物标记。使用非靶向方法，我们还检测了被鉴定为阿魏酸和咖啡酸硫酸盐的酚类共轭物，以及支持I相和（或）微生物酶进一步类脂多糖类代谢的羟苄基和羟苯基乙醛硫酸盐衍生物。总体而言，这项研究的结果表明，健康人志愿者中从水曲柳提取物中完整的类蛇形素的摄取量低，并且酯酶，糖苷酶以及II期磺基和葡萄糖醛糖基转移酶代谢转化形成较小的共轭衍生物。

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