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首页> 外文期刊>Nucleic acids research >Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae
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Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae

机译：插入序列的大小和位置对于酿酒酵母中pre-mRNA的剪接效率至关重要。

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摘要

The size of the 309 bp actin gene Intron of the yeast Saccharomyces cerevisiae was enlarged by inserting DNA fragments of different lengths and sequence. Enlarging the intron above 551 bp, the largest known yeast intron, led to a decrease in splicing efficiency. The effect on transcript splicing was dependent on the length of the inserted fragments rather then their sequence. It was also observed that insertion of the actin gene intron into different regions of the normally unsplit yeast YP2 gene, significantly influenced the efficiency of splicing of the resulting transcripts. The splicing efficiency decreased with the increase of the distance between the mRNA cap site and the intervening sequence
机译：通过插入不同长度和序列的DNA片段，扩大了酿酒酵母309 bp肌动蛋白基因内含子的大小。将内含子增加到551 bp以上，这是已知的最大的酵母内含子，导致剪接效率降低。对转录剪接的影响取决于插入片段的长度，而不是其序列。还观察到肌动蛋白基因内含子插入通常未分裂的酵母YP2基因的不同区域，显着影响了所得转录物的剪接效率。剪接效率随着mRNA帽位点与插入序列之间距离的增加而降低

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